Human Recombinant F13 protein, His Tag (V2LY-0526-LY3995)

Name: Human Recombinant F13 protein, His Tag
SKU: V2LY-0526-LY3995
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant F13 protein, His Tag consist of Amino Acid: 1-661 and predicts a molecular mass of 74.5 kDa.

Molecule Mass

74.5 kDa

Verified

HPLC

Sequence

Amino Acid: 1-661

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Coagulation Factor XIII A Chain

Research Area

Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

Biological Process

Blood coagulation Source: ProtInc
Blood coagulation, fibrin clot formation Source: UniProtKB
Peptide cross-linking Source: UniProtKB

Cellular Location

Secreted; Cytoplasm. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.

Involvement in disease

Factor XIII subunit A deficiency (FA13AD):
An autosomal recessive hematologic disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.

PTM

The activation peptide is released by thrombin.

Xie, H., Wang, M., Jin, Y., Li, X., Jiang, S., & Yang, L. (2022). A novel F13A1 gene mutation (Arg208Pro) in a Chinese patient with factor XIII deficiency. Blood Coagulation & Fibrinolysis, 33(6), 337-341.

Katsaras, G. N., Gialamprinou, D., Chatziioannidis, I., Karagianni, P., & Mitsiakos, G. (2022). Coagulation factor XIII deficiency–Report of a newborn F13A1 Val34Leu polymorphism carrier. Journal of Pediatric and Neonatal Individualized Medicine (JPNIM), 11(2), e110238-e110238.

Kaartinen, M. T., Arora, M., Heinonen, S., Hang, A., Barry, A., Lundbom, J., ... & Pietiläinen, K. H. (2021). F13A1 transglutaminase expression in human adipose tissue increases in acquired excess weight and associates with inflammatory status of adipocytes. International Journal of Obesity, 45(3), 577-587.

Ercan, H., Mauracher, L. M., Grilz, E., Hell, L., Hellinger, R., Schmid, J. A., ... & Zellner, M. (2021). Alterations of the platelet proteome in lung cancer: Accelerated F13A1 and ER processing as new actors in hypercoagulability. Cancers, 13(9), 2260.

Kaartinen, M. T., Arora, M., Heinonen, S., Rissanen, A., Kaprio, J., & Pietiläinen, K. H. (2020). Transglutaminases and Obesity in Humans: Association of F13A1 to Adipocyte Hypertrophy and Adipose Tissue Immune Response. International journal of molecular sciences, 21(21), 8289.

Songdej, N., Del Carpio-cano, F., Mao, G., Wurtzel, J., Goldfinger, L., Lambert, M. P., & Rao, A. K. (2020). Transcription factor RUNX1 regulates factor FXIIIA subunit (F13A1) expression in megakaryocytic cells and platelet F13A1 expression is downregulated in RUNX1 haplodeficiency. Blood, 136, 25-26.

Ma, S., Chen, C., Liang, Q., Wu, X., Wang, X., Wu, W., ... & Ding, Q. (2019). Phenotype and genotype of FXIII deficiency in two unrelated probands: identification of a novel F13A1 large deletion mediated by complex rearrangement. Orphanet journal of rare diseases, 14(1), 1-10.

Ansani, L., Marchesini, J., Pestelli, G., Luisi, G. A., Scillitani, G., Longo, G., ... & Gemmati, D. (2018). F13A1 gene variant (V34L) and residual circulating FXIIIA levels predict short-and long-term mortality in acute myocardial infarction after coronary angioplasty. International journal of molecular sciences, 19(9), 2766.

Gemmati, D., Occhionorelli, S., Tisato, V., Vigliano, M., Longo, G., Gonelli, A., ... & Zamboni, P. (2018). Inherited genetic predispositions in F13A1 and F13B genes predict abdominal adhesion formation: Identification of gender prognostic indicators. Scientific reports, 8(1), 1-13.

Peltier, J., Roperch, J. P., Audebert, S., Borg, J. P., & Camoin, L. (2018). Activation peptide of the coagulation factor XIII (AP-F13A1) as a new biomarker for the screening of colorectal cancer. Clinical proteomics, 15(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

F13A1 Matched Antibody Pair (395) (CAT#: APMAB-395LY)

Antibody Pairs

F13A1 Matched Antibody Pair (395) (CAT#: APMAB-395LY)

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Isotype control

For research use only. Not intended for any clinical use.

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