Human Recombinant FLRT2 protein, His Tag (V2LY-0526-LY4154)

Name: Human Recombinant FLRT2 protein, His Tag
SKU: V2LY-0526-LY4154
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant FLRT2 protein, His Tag consist of Amino Acid: 1-539 and predicts a molecular mass of 57.7 kDa.

Molecule Mass

57.7 kDa

Sequence

Amino Acid: 1-539

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>98% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile NaCl, KCl, Na2HPO4, KH2PO4, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Fibronectin Leucine Rich Transmembrane Protein 2

Function

Functions in cell-cell adhesion, cell migration and axon guidance. Mediates cell-cell adhesion via its interactions with ADGRL3 and probably also other latrophilins that are expressed at the surface of adjacent cells. May play a role in the migration of cortical neurons during brain development via its interaction with UNC5D. Mediates axon growth cone collapse and plays a repulsive role in neuron guidance via its interaction with UNC5D, and possibly also other UNC-5 family members. Plays a role in fibroblast growth factor-mediated signaling cascades. Required for normal organization of the cardiac basement membrane during embryogenesis, and for normal embryonic epicardium and heart morphogenesis.

Biological Process

Axon guidance Source: Ensembl
Basement membrane organization Source: UniProtKB
Cell adhesion involved in heart morphogenesis Source: UniProtKB
Fibroblast growth factor receptor signaling pathway Source: UniProtKB
Heart morphogenesis Source: UniProtKB
Positive regulation of synapse assembly Source: Ensembl
Regulation of neuron migration Source: Ensembl

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane; Cell membrane; Extracellular matrix; Secreted; Focal adhesion; Synaptosome. Proteolytic cleavage gives rise to a shedded ectodomain.

Topology

Extracellular: 36-541
Helical: 542-562
Cytoplasmic: 563-660

PTM

N-glycosylated.
Proteolytic cleavage in the juxtamembrane region gives rise to a soluble ectodomain. Cleavage is probably effected by a metalloprotease.

Ando, T., Tai-Nagara, I., Sugiura, Y., Kusumoto, D., Okabayashi, K., Kido, Y., ... & Kubota, Y. (2022). Tumor-specific interendothelial adhesion mediated by FLRT2 facilitates cancer aggressiveness. The Journal of clinical investigation, 132(6).

Prigge, C. L., Sharma, A., Dembla, M., El-Quessny, M., Kozlowski, C., Paisley, C. E., ... & Kay, J. N. (2022). Rejection of inappropriate synaptic partners mediated by transcellular FLRT2-UNC5 signaling. bioRxiv, 2022-08.

Jackson, V., Hermann, J., Tynan, C. J., Rolfe, D. J., Corey, R. A., Duncan, A. L., ... & Chavent, M. (2022). The guidance and adhesion protein FLRT2 dimerizes in cis via dual Small-X3-Small transmembrane motifs. Structure, 30(9), 1354-1365.

Fleitas, C., Marfull-Oromí, P., Chauhan, D., Del Toro, D., Peguera, B., Zammou, B., ... & Egea, J. (2021). FLRT2 and FLRT3 cooperate in maintaining the tangential migratory streams of cortical interneurons during development. Journal of Neuroscience, 41(35), 7350-7362.

Li, J., Shinoda, Y., Ogawa, S., Ikegaya, S., Li, S., Matsuyama, Y., ... & Yamagishi, S. (2021). Expression of FLRT2 in Postnatal Central Nervous System Development and After Spinal Cord Injury. Frontiers in Molecular Neuroscience, 241.

Fleitas Pérez, C., Marfull Oromí, P., Chauhan, D., Toro, D. D., Peguera, B., Zammou, B., ... & Egea Navarro, J. (2021). FLRT2 and FLRT3 Cooperate in Maintaining the Tangential Migratory Streams of Cortical Interneurons during Development. Journal of Neuroscience, 2021, vol. 41, núm. 35, 7350-7362.

Guo, X., Song, C., Fang, L., Li, M., Yue, L., & Sun, Q. (2020). FLRT2 functions as Tumor Suppressor gene inactivated by promoter methylation in Colorectal Cancer. Journal of Cancer, 11(24), 7329.

Shirakawa, J., Takegahara, N., Kim, H., Lee, S. H., Sato, K., Yamagishi, S., & Choi, Y. (2019). Flrt2 is involved in fine-tuning of osteoclast multinucleation. BMB reports, 52(8), 514.

Cicvaric, A., Yang, J., Bulat, T., Zambon, A., Dominguez-Rodriguez, M., Kühn, R., ... & Monje, F. J. (2018). Enhanced synaptic plasticity and spatial memory in female but not male FLRT2-haplodeficient mice. Scientific Reports, 8(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant FLRT2 protein, His Tag (CAT#: V2LY-0526-LY8331)

Related Products

Mouse Anti-FLRT2 Recombinant Antibody (CBXF-3341) (CAT#: CBMAB-F0662-CQ)

Mouse Recombinant FLRT2 protein, His Tag (CAT#: V2LY-0526-LY8331)

Isotype control

For research use only. Not intended for any clinical use.

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