Human Recombinant FUCA1 protein, His Tag (V2LY-0526-LY4220)

Name: Human Recombinant FUCA1 protein, His Tag
SKU: V2LY-0526-LY4220
Rating: 5 (1 reviews)

See all FUCA1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant FUCA1 protein, His Tag consist of Amino Acid: 32-466 and predicts a molecular mass of 51.9 kDa.

Molecule Mass

51.9 kDa

Sequence

Amino Acid: 32-466

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Alpha-L-Fucosidase 1

Function

Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins.

Biological Process

Fucose metabolic process Source: UniProtKB
Glycolipid catabolic process Source: BHF-UCL
Glycosaminoglycan catabolic process Source: UniProtKB
Glycoside catabolic process Source: UniProtKB

Cellular Location

Lysosome

Involvement in disease

Fucosidosis (FUCA1D):
An autosomal recessive lysosomal storage disease characterized by accumulation of fucose-containing glycolipids and glycoproteins in various tissues. Clinical signs include facial dysmorphism, dysostosis multiplex, moderate hepatomegaly, severe intellectual deficit, deafness, and according to age, angiokeratomas.

Xiao, Y., Jiang, X., Yin, K., Miao, T., Lu, H., Wang, W., ... & Zhang, P. (2023). USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer. Oncogenesis, 12(1), 12.

Armstrong, Z., Meek, R. W., Wu, L., Blaza, J. N., & Davies, G. J. (2022). Cryo-EM structures of human fucosidase FucA1 reveal insight into substrate recognition and catalysis. Structure, 30(10), 1443-1451.

Barelier, S., & Sulzenbacher, G. (2022). The long-awaited structure of human fucosidase FucA1 opens novel avenues for the treatment of fucosidosis. Structure, 30(10), 1369-1371.

Chkioua, L., Amri, Y., Saheli, C., Fenni, F., Boudabous, H., Ben Turkia, H., ... & Laradi, S. (2021). Fucosidosis in Tunisian patients: mutational analysis and homology-based modeling of FUCA1 enzyme. BMC medical genomics, 14, 1-11.

Zhang, X., Zhao, S., Liu, H., Wang, X., Wang, X., Du, N., ... & Duan, H. (2021). Identification of a novel homozygous loss-of-function mutation in FUCA1 gene causing severe fucosidosis: A case report. Journal of International Medical Research, 49(4), 03000605211005975.

Domin, A., Zabek, T., Kwiatkowska, A., Szmatola, T., Deregowska, A., Lewinska, A., ... & Wnuk, M. (2021). The Identification of a Novel Fucosidosis-Associated FUCA1 Mutation: A Case of a 5-Year-Old Polish Girl with Two Additional Rare Chromosomal Aberrations and Affected DNA Methylation Patterns. Genes, 12(1), 74.

Wali, G., Wali, G. M., Sue, C. M., & Kumar, K. R. (2019). A novel homozygous mutation in the FUCA1 gene highlighting fucosidosis as a cause of dystonia: Case report and literature review. Neuropediatrics, 50(04), 248-252.

Bonin, S., Parascandolo, A., Aversa, C., Barbazza, R., Tsuchida, N., Castellone, M. D., ... & Vecchio, G. (2018). Reduced expression of α-L-Fucosidase-1 (FUCA-1) predicts recurrence and shorter cancer specific survival in luminal B LN+ breast cancer patients. Oncotarget, 9(20), 15228.

Valero‐Rubio, D., Jiménez, K. M., Fonseca, D. J., Payán‐Gómez, C., & Laissue, P. (2018). Transcriptomic analysis of FUCA 1 knock‐down in keratinocytes reveals new insights into the pathogenesis of fucosidosis skin lesions. Experimental dermatology, 27(6), 663-667.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant FUCA1 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

FUCA1 Matched Antibody Pair (456) (CAT#: APMAB-456LY)

Antibody Pairs

FUCA1 Matched Antibody Pair (456) (CAT#: APMAB-456LY)

Related Products

Mouse Anti-FUCA1 Recombinant Antibody (1D4) (CAT#: CBMAB-A4409-YC)

Mouse Anti-FUCA1 Recombinant Antibody (CBYC-A958) (CAT#: V2LY-1225-LY252)

Mouse Anti-FUCA1 Recombinant Antibody (CBXF-0955) (CAT#: CBMAB-F0992-CQ)

Mouse Anti-FUCA1 Recombinant Antibody (CBXF-0955) (CAT#: V2LY-1225-LY250)

Rabbit Anti-FUCA1 Recombinant Antibody (CBXF-1101) (CAT#: CBMAB-F0993-CQ)

Mouse Anti-FUCA1 Recombinant Antibody (CBYC-A958) (CAT#: CBMAB-A4411-YC)

Mouse Anti-FUCA1 Recombinant Antibody (CBXF-3232) (CAT#: CBMAB-F3927-CQ)

Mouse Anti-FUCA1 Recombinant Antibody (CBYC-A959) (CAT#: CBMAB-A4412-YC)

FUCA1 Matched Antibody Pair (456) (CAT#: APMAB-456LY)

Mouse Anti-FUCA1 Recombinant Antibody (CBYJT-1284) (CAT#: CBMAB-T0299-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry