Human Recombinant FZD5 protein, ECD, hFc Tag (V2LY-0526-LY4209)

Name: Human Recombinant FZD5 protein, ECD, hFc Tag
SKU: V2LY-0526-LY4209
Rating: 5 (1 reviews)

See all FZD5 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant FZD5 protein, ECD, hFc Tag consist of Amino Acid: 1-167 and predicts a molecular mass of 42.7 kDa.

Molecule Mass

42.7 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-167

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

frizzled class receptor 5

Function

Receptor for Wnt proteins (PubMed:9054360, PubMed:10097073, PubMed:20530549).

Can activate WNT2, WNT10B, WNT5A, but not WNT2B or WNT4 (in vitro); the in vivo situation may be different since not all of these are known to be coexpressed (By similarity).

In neurons, activation of WNT7A promotes formation of synapses (PubMed:20530549).

Functions in the canonical Wnt/beta-catenin signaling pathway. The canonical Wnt/beta-catenin signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes (By similarity).

A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (Probable). Plays a role in yolk sac angiogenesis and in placental vascularization (By similarity).

Biological Process

Angiogenesis Source: Ensembl
Anterior/posterior axis specification, embryo Source: BHF-UCL
Apoptotic process involved in morphogenesis Source: Ensembl
Branching involved in labyrinthine layer morphogenesis Source: Ensembl
Canonical Wnt signaling pathway Source: BHF-UCL
Cell maturation Source: Ensembl
Cellular response to molecule of bacterial origin Source: BHF-UCL
Chorionic trophoblast cell differentiation Source: Ensembl
Embryonic axis specification Source: BHF-UCL
Embryonic camera-type eye morphogenesis Source: Ensembl
Labyrinthine layer blood vessel development Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
Neuron differentiation Source: UniProtKB
Positive regulation of interferon-gamma production Source: BHF-UCL
Positive regulation of interleukin-1 beta production Source: ARUK-UCL
Positive regulation of JUN kinase activity Source: Ensembl
Positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
Positive regulation of T cell cytokine production Source: CACAO
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of tumor necrosis factor production Source: ARUK-UCL
Post-embryonic camera-type eye development Source: Ensembl
Regulation of autophagy of mitochondrion Source: ParkinsonsUK-UCL
Regulation of bicellular tight junction assembly Source: Ensembl
Regulation of chorionic trophoblast cell proliferation Source: Ensembl
Spemann organizer formation Source: BHF-UCL
Synapse assembly Source: ParkinsonsUK-UCL
Syncytiotrophoblast cell differentiation involved in labyrinthine layer development Source: Ensembl
T cell differentiation in thymus Source: Ensembl

Cellular Location

Golgi apparatus membrane; Cell membrane; Synapse; Perikaryon; Dendrite; Axon. Localized at the plasma membrane and also found at the Golgi.

Topology

Extracellular: 27-238
Helical: 239-259
Cytoplasmic: 260-270
Helical: 271-291
Extracellular: 292-315
Helical: 316-336
Cytoplasmic: 337-358
Helical: 359-379
Extracellular: 380-402
Helical: 403-423
Cytoplasmic: 424-449
Helical: 450-470
Extracellular: 471-500
Helical: 501-521
Cytoplasmic: 522-585

PTM

Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.

Cao, G., Long, K., Qiu, Y., Ma, Y., Qin, H., Huo, D., ... & Hou, C. (2023). Inducible positive amplification regulation coupled with the double-strand specific nuclease for FzD5 mRNA assay. Microchemical Journal, 184, 108179.

Holt, R., Goudie, D., Verde, A. D., Gardham, A., Ramond, F., Putoux, A., ... & Ragge, N. K. (2022). Individuals with heterozygous variants in the Wnt-signalling pathway gene FZD5 delineate a phenotype characterized by isolated coloboma and variable expressivity. Ophthalmic Genetics, 43(6), 809-816.

Peng, N., Zhang, Z., Wang, Y., Yang, M., Fan, J., Wang, Q., ... & Li, W. (2021). Down‐regulated LINC00115 inhibits prostate cancer cell proliferation and invasion via targeting miR‐212‐5p/FZD5/Wnt/β‐catenin axis. Journal of Cellular and Molecular Medicine, 25(22), 10627-10637.

Aubert-Mucca, M., Pernin-Grandjean, J., Marchasson, S., Gaston, V., Habib, C., Meunier, I., ... & Plaisancié, J. (2021). Confirmation of FZD5 implication in a cohort of 50 patients with ocular coloboma. European Journal of Human Genetics, 29(1), 131-140.

Liu, X., Li, Y., Chen, C., & Li, L. (2020). miR-149 regulates the proliferation and apoptosis of human colonic carcinoma cells by targeting FZD5. International Journal of Clinical and Experimental Pathology, 13(5), 889.

Thiele, S., Zimmer, A., Göbel, A., Rachner, T. D., Rother, S., Fuessel, S., ... & Hofbauer, L. C. (2018). Role of WNT5A receptors FZD5 and RYK in prostate cancer cells. Oncotarget, 9(43), 27293.

Dai, Y., Lu, H., Wang, S., Chang, S., Li, C., Huang, Z., ... & Ge, J. (2018). MicroRNA-216b actively modulates diabetic angiopathy through inverse regulation on FZD5. Gene, 658, 129-135.

Lin, J. F., Zeng, H., & Zhao, J. Q. (2018). MiR-212-5p regulates the proliferation and apoptosis of AML cells through targeting FZD5. Eur Rev Med Pharmacol Sci, 22(23), 8415-8422.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant FZD5 protein, ECD, hFc Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant FZD5 protein, His Tag (CAT#: V2LY-0526-LY4210)

Mouse Recombinant FZD5 protein, hFc Tag (CAT#: V2LY-0526-LY8345)

Related Products

Mouse Anti-FZD5 Recombinant Antibody (CBXF-0991) (CAT#: CBMAB-F1010-CQ)

Mouse Anti-FZD5 Recombinant Antibody (6A3) (CAT#: CBMAB-A3255-LY)

Human Recombinant FZD5 protein, His Tag (CAT#: V2LY-0526-LY4210)

Rabbit Anti-FZD5 Recombinant Antibody (EG1256) (CAT#: CBMAB-EN1519-LY)

Mouse Anti-FZD5 Recombinant Antibody (CBXF-1820) (CAT#: V2LY-1225-LY406)

Mouse Recombinant FZD5 protein, hFc Tag (CAT#: V2LY-0526-LY8345)

Mouse Anti-FZD5 Recombinant Antibody (CBXF-2374) (CAT#: CBMAB-F1623-CQ)

Mouse Anti-FZD5 Recombinant Antibody (CBXF-0990) (CAT#: V2LY-1225-LY404)

Rabbit Anti-FZD5 Recombinant Antibody (CBXF-2087) (CAT#: CBMAB-F2036-CQ)

Rabbit Anti-FZD5 Recombinant Antibody (CBXF-2087) (CAT#: V2LY-1225-LY405)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry