Human Recombinant GABARAP protein, His & MBP Tag (V2LY-0526-LY4232)

Name: Human Recombinant GABARAP protein, His & MBP Tag
SKU: V2LY-0526-LY4232
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His & MBP Tag

Protein Construction

This product is Human Recombinant GABARAP protein, His & MBP Tag consist of Amino Acid: 1-117 and predicts a molecular mass of 57.5 kDa.

Molecule Mass

57.5 kDa

Sequence

Amino Acid: 1-117

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

GABA Type A Receptor-Associated Protein

Function

Ubiquitin-like modifier that plays a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton (PubMed:9892355).

Involved in autophagy: while LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:15169837, PubMed:20562859, PubMed:22948227).

Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006538).

Also required for the local activition of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, regulating ubiquitination and degradation of TIAM1, a guanyl-nucleotide exchange factor (GEF) that activates RAC1 and downstream signal transduction (PubMed:25684205).

Thereby, regulates different biological processes including the organization of the cytoskeleton, cell migration and proliferation (PubMed:25684205).

Involved in apoptosis (PubMed:15977068).

Biological Process

Autophagosome assembly Source: GO_Central
Autophagy of mitochondrion Source: GO_Central
Cellular response to nitrogen starvation Source: GO_Central
Chemical synaptic transmission Source: ProtInc
Extrinsic apoptotic signaling pathway via death domain receptors Source: UniProtKB
Macroautophagy Source: GO_Central
Microtubule cytoskeleton organization Source: Ensembl
Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Positive regulation of protein K48-linked ubiquitination Source: UniProtKB
Protein targeting Source: ProtInc
Regulation of Rac protein signal transduction Source: UniProtKB

Cellular Location

Golgi apparatus membrane; Cytoskeleton; Endomembrane system; Autophagosome; Cytoplasmic vesicle. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).

PTM

The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAP-I (PubMed:15169837). This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAP-II (PubMed:15169837).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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