Human Recombinant GAD67 protein, His Tag (V2LY-0526-LY4236)

Name: Human Recombinant GAD67 protein, His Tag
SKU: V2LY-0526-LY4236
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GAD67 protein, His Tag consist of Amino Acid: 1-594 and predicts a molecular mass of 68.3 kDa.

Molecule Mass

68.3 kDa

Sequence

Amino Acid: 1-594

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>75% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Glutamate Decarboxylase 1

Function

Catalyzes the production of GABA.

Biological Process

Chemical synaptic transmission Source: ProtInc
Gamma-aminobutyric acid biosynthetic process Source: Ensembl
Glutamate catabolic process Source: UniProtKB
Glutamate decarboxylation to succinate Source: ProtInc
Locomotory exploration behavior Source: Ensembl
Neurotransmitter biosynthetic process Source: UniProtKB-KW
Protein-pyridoxal-5-phosphate linkage Source: UniProtKB
Response to drug Source: Ensembl
Social behavior Source: Ensembl

Cellular Location

Plasma membrane; Axon terminus; Cell cortex; Clathrin-sculpted gamma-aminobutyric acid transport vesicle; Inhibitory synapse; Presynaptic active zone; Vesicle membrane

Involvement in disease

Cerebral palsy, spastic quadriplegic 1 (CPSQ1):
A non-progressive disorder of movement and/or posture resulting from defects in the developing central nervous system. Affected individuals manifest symmetrical, non-progressive spasticity and no adverse perinatal history or obvious underlying alternative diagnosis. Developmental delay, mental retardation and sometimes epilepsy can be part of the clinical picture.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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