Human Recombinant GNRH2 protein, hFc Tag (V2LY-0526-LY4378)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Human
Tag
hFc Tag
Protein Construction
This product is Human Recombinant GNRH2 protein, hFc Tag consist of Amino Acid: 24-120 and predicts a molecular mass of 39 kDa.
Molecule Mass
39 kDa
Sequence
Amino Acid: 24-120
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
Gonadotropin Releasing Hormone 2
Function
Stimulates the secretion of gonadotropins; it stimulates the secretion of both luteinizing and follicle-stimulating hormones.
Biological Process
Reproduction Source: GO_Central
Signal transduction Source: ProtInc
Cellular Location
Secreted

Sun, A., Yan, X., Sun, J., Tian, Z., Wang, W., & Hu, H. (2022). Presence of GnRH3 in sturgeon and the roles of GnRH1 and GnRH2 on the regulation of LH/FSH in mature female sterlet Acipenser ruthenus in vitro. General and Comparative Endocrinology, 323, 114026.

Kumari, P., Kumar, M., Sehgal, N., & Aggarwal, N. (2022). In silico analysis of kiss2, expression studies and protein–protein interaction with gonadotropin-releasing hormone 2 (GnRH2) and luteinizing hormone beta (LHβ) in Heteropneustes fossilis. Journal of Biomolecular Structure and Dynamics, 40(10), 4543-4557.

Marvel, M., Levavi-Sivan, B., Wong, T. T., Zmora, N., & Zohar, Y. (2021). Gnrh2 maintains reproduction in fasting zebrafish through dynamic neuronal projection changes and regulation of gonadotropin synthesis, oogenesis, and reproductive behaviors. Scientific Reports, 11(1), 1-16.

Yuan, D., Wang, B., Tang, T., Lei, L., Zhou, C., Li, Z., & Li, L. (2021). Characterization and evaluation of the tissue distribution of CRH, apelin, and GnRH2 reveal responses to feeding states in Schizothorax davidi. Fish Physiology and Biochemistry, 47, 421-438.

Feng, W., Wang, Z., Feng, D., Zhu, Y., Zhang, K., & Huang, W. (2020). The effects of common variants in MDM2 and GNRH2 genes on the risk and survival of osteosarcoma in Han populations from Northwest China. Scientific Reports, 10(1), 15939.

Wang, W., Chen, J., Fang, Y., Wang, B., Zou, Q., Wang, L., ... & Wang, K. (2019). Identification of gnrh2 and gnrh3 and their expression during brood pouch growth and short-term benzo (a) pyrene exposure in lined seahorse (Hippocampus erectus). Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 225, 108579.

Marvel, M. M., Spicer, O. S., Wong, T. T., Zmora, N., & Zohar, Y. (2019). Knockout of Gnrh2 in zebrafish (Danio rerio) reveals its roles in regulating feeding behavior and oocyte quality. General and Comparative Endocrinology, 280, 15-23.

Ding, H., Liu, M., Zhou, C., You, X., Suo, Z., Zhang, C., & Xu, D. (2019). Expression and regulation of GnRHR2 gene and testosterone secretion mediated by GnRH2 and GnRHR2 within porcine testes. The Journal of Steroid Biochemistry and Molecular Biology, 190, 161-172.

Feng, K., Luo, H., Hou, M., Li, Y., Chen, J., Zhu, Z., & Hu, W. (2018). Alternative splicing of GnRH2 and GnRH2-associated peptide plays roles in gonadal differentiation of the rice field eel, Monopterus albus. General and Comparative Endocrinology, 267, 9-17.

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For research use only. Not intended for any clinical use.

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