Human Recombinant GOLM1 protein, His Tag (V2LY-0526-LY4379)

Name: Human Recombinant GOLM1 protein, His Tag
SKU: V2LY-0526-LY4379
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GOLM1 protein, His Tag consist of Amino Acid: 40-401 and predicts a molecular mass of 42.7 kDa.

Molecule Mass

42.7 kDa

Sequence

Amino Acid: 40-401

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Golgi Membrane Protein 1

Function

Unknown. Cellular response protein to viral infection.

Biological Process

Nucleus organization Source: Ensembl
Regulation of lipid metabolic process Source: Ensembl

Cellular Location

Cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.

Topology

Cytoplasmic: 1-12
Helical: 13-35
Lumenal: 36-401

PTM

Glycosylated.
Phosphorylation sites are present in the extracellular medium.

Shao, W. Q., Zhu, W. W., Luo, M. J., Fan, M. H., Li, Q., Wang, S. H., ... & Qin, L. X. (2022). Cholesterol suppresses GOLM1-dependent selective autophagy of RTKs in hepatocellular carcinoma. Cell Reports, 39(3), 110712.

Sun, Z., Mao, Y., Zhang, X., Lu, S., Wang, H., Zhang, C., ... & Li, K. (2021). Identification of ARHGEF38, NETO2, GOLM1, and SAPCD2 associated with prostate cancer progression by bioinformatic analysis and experimental validation. Frontiers in Cell and Developmental Biology, 9, 718638.

Chen, J., Lin, Z., Liu, L., Zhang, R., Geng, Y., Fan, M., ... & Qin, L. X. (2021). GOLM1 exacerbates CD8+ T cell suppression in hepatocellular carcinoma by promoting exosomal PD-L1 transport into tumor-associated macrophages. Signal transduction and targeted therapy, 6(1), 397.

Pu, Y., Song, Y., Zhang, M., Long, C., Li, J., Wang, Y., ... & Zhang, H. (2021). GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis. Signal Transduction and Targeted Therapy, 6(1), 148.

Yan, J., Zhou, B., Li, H., Guo, L., & Ye, Q. (2020). Recent advances of GOLM1 in hepatocellular carcinoma. Hepatic oncology, 7(2), HEP22.

Yan, J., Zhou, B., Guo, L., Chen, Z., Zhang, B., Liu, S., ... & Ye, Q. (2020). GOLM1 upregulates expression of PD-L1 through EGFR/STAT3 pathway in hepatocellular carcinoma. American journal of cancer research, 10(11), 3705.

Lin, Y., Dong, H., Deng, W., Lin, W., Li, K., Xiong, X., ... & Zhang, H. (2019). Evaluation of Salivary Exosomal Chimeric GOLM1-NAA35 RNA as a Potential Biomarker in Esophageal CarcinomaExosomal Chimeric RNA in Cancer. Clinical Cancer Research, 25(10), 3035-3045.

Teerlink, C. C., Huff, C., Stevens, J., Yu, Y., Holmen, S. L., Silvis, M. R., ... & Cannon-Albright, L. A. (2018). A nonsynonymous variant in the GOLM1 gene in cutaneous malignant melanoma. JNCI: Journal of the National Cancer Institute, 110(12), 1380-1385.

Yan, G., Ru, Y., Wu, K., Yan, F., Wang, Q., Wang, J., ... & Zou, L. (2018). GOLM1 promotes prostate cancer progression through activating PI3K‐AKT‐mTOR signaling. The Prostate, 78(3), 166-177.

Yang, L., Luo, P., Song, Q., & Fei, X. (2018). DNMT1/miR-200a/GOLM1 signaling pathway regulates lung adenocarcinoma cells proliferation. Biomedicine & Pharmacotherapy, 99, 839-847.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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