Human Recombinant GZMA protein, His Tag (V2LY-0526-LY4407)

Name: Human Recombinant GZMA protein, His Tag
SKU: V2LY-0526-LY4407
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant GZMA protein, His Tag consist of Amino Acid: 1-262 and predicts a molecular mass of 27.5 kDa.

Molecule Mass

27.5 kDa

Sequence

Amino Acid: 1-262

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

granzyme A

Function

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed:3257574, PubMed:3262682, PubMed:3263427, PubMed:32299851, PubMed:12819770).

It cleaves after Lys or Arg (PubMed:32299851, PubMed:12819770).

Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-B (GSDMB), releasing the pore-forming moiety of GSDMB, thereby triggering pyroptosis and target cell death (PubMed:32299851).

Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity (PubMed:12524539).

Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA (PubMed:11555662, PubMed:12628186, PubMed:16818237).

Biological Process

Apoptotic process Source: UniProtKB
Cytolysis Source: UniProtKB-KW
Cytotoxic T cell pyroptotic process Source: UniProtKB
Granzyme-mediated programmed cell death signaling pathway Source: UniProtKB
Immune response Source: UniProtKB
Negative regulation of DNA binding Source: UniProtKB
Negative regulation of endodeoxyribonuclease activity Source: UniProtKB
Negative regulation of oxidoreductase activity Source: UniProtKB
Positive regulation of apoptotic process Source: UniProtKB
Proteolysis involved in cellular protein catabolic process Source: UniProtKB
Pyroptosis Source: UniProtKB
Response to bacterium Source: Ensembl

Cellular Location

Isoform alpha: Secreted; Cytoplasmic granule. Delivered into the target cell by perforin.

Cheng, Z., Thompson, E. J., Mendive‐Tapia, L., Scott, J. I., Benson, S., Kitamura, T., ... & Vendrell, M. (2023). Fluorogenic Granzyme A Substrates Enable Real‐Time Imaging of Adaptive Immune Cell Activity. Angewandte Chemie, 135(8), e202216142.

Moreno-Martinez, L., Santiago, L., de la Torre, M., Calvo, A. C., Pardo, J., & Osta, R. (2022). Hemizygous Granzyme A Mice Expressing the hSOD1G93A Transgene Show Slightly Extended Lifespan. International Journal of Molecular Sciences, 23(21), 13554.

Rawle, D. J., Le, T. T., Dumenil, T., Bishop, C., Yan, K., Nakayama, E., ... & Suhrbier, A. (2022). Widespread discrepancy in Nnt genotypes and genetic backgrounds complicates granzyme A and other knockout mouse studies. Elife, 11, e70207.

Garzón-Tituaña, M., Sierra-Monzón, J. L., Comas, L., Santiago, L., Khaliulina-Ushakova, T., Uranga-Murillo, I., ... & Arias, M. (2021). Granzyme A inhibition reduces inflammation and increases survival during abdominal sepsis. Theranostics, 11(8), 3781.

Di Censo, C., Marotel, M., Mattiola, I., Müller, L., Scarno, G., Pietropaolo, G., ... & Sciumè, G. (2021). Granzyme A and CD160 expression delineates ILC1 with graded functions in the mouse liver. European Journal of Immunology, 51(11), 2568-2575.

Santiago, L., Castro, M., Sanz-Pamplona, R., Garzon, M., Ramirez-Labrada, A., Tapia, E., ... & Pardo, J. (2020). Extracellular granzyme A promotes colorectal cancer development by enhancing gut inflammation. Cell reports, 32(1).

Zhou, Z., He, H., Wang, K., Shi, X., Wang, Y., Su, Y., ... & Shao, F. (2020). Granzyme A from cytotoxic lymphocytes cleaves GSDMB to trigger pyroptosis in target cells. Science, 368(6494), eaaz7548.

Schanoski, A. S., Le, T. T., Kaiserman, D., Rowe, C., Prow, N. A., Barboza, D. D., ... & Suhrbier, A. (2020). Granzyme A in chikungunya and other arboviral infections. Frontiers in immunology, 10, 3083.

Shimizu, K., Yamasaki, S., Sakurai, M., Yumoto, N., Ikeda, M., Mishima-Tsumagari, C., ... & Fujii, S. I. (2019). Granzyme A stimulates pDCs to promote adaptive immunity via induction of type I IFN. Frontiers in immunology, 10, 1450.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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