Human Recombinant HBEGF protein (V2LY-0526-LY4459)

Name: Human Recombinant HBEGF protein
SKU: V2LY-0526-LY4459
Rating: 5 (1 reviews)

See all HBEGF antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Protein Construction

This product is Human Recombinant HBEGF protein consist of Amino Acid: 1-148 and predicts a molecular mass of 16.4 kDa.

Molecule Mass

16.4 kDa

Sequence

Amino Acid: 1-148

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

heparin-binding EGF-like growth factor

Function

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Biological Process

Cell chemotaxis Source: UniProtKB
Epidermal growth factor receptor signaling pathway Source: BHF-UCL
Muscle organ development Source: ProtInc
Negative regulation of elastin biosynthetic process Source: Ensembl
Positive regulation of cell growth Source: Ensembl
Positive regulation of cell migration Source: BHF-UCL
Positive regulation of cell population proliferation Source: GO_Central
Positive regulation of epidermal growth factor-activated receptor activity Source: GO_Central
Positive regulation of keratinocyte migration Source: Ensembl
Positive regulation of protein kinase B signaling Source: BHF-UCL
Positive regulation of smooth muscle cell proliferation Source: Ensembl
Positive regulation of wound healing Source: BHF-UCL
Regulation of heart contraction Source: Ensembl
Signal transduction Source: ProtInc
Wound healing, spreading of epidermal cells Source: Ensembl

Cellular Location

Heparin-binding EGF-like growth factor: Extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
Proheparin-binding EGF-like growth factor: Cell membrane

Topology

Extracellular: 20-160
Helical: 161-184
Cytoplasmic: 185-208

PTM

Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-glycosylated with core 1 or possibly core 8 glycans. Thr-47 is a minor glycosylation site compared to Thr-44.

Kim, Y. S., Yuan, J., Dewar, A., Borg, J. P., Threadgill, D. W., Sun, X., & Dey, S. K. (2023). An unanticipated discourse of HB-EGF with VANGL2 signaling during embryo implantation. Proceedings of the National Academy of Sciences, 120(20), e2302937120.

Zhou, L., Zhou, W., Joseph, A. M., Chu, C., Putzel, G. G., Fang, B., ... & Sonnenberg, G. F. (2022). Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation. Nature immunology, 23(2), 251-261.

Yi, L., Ai, K., Li, H., Qiu, S., Li, Y., Wang, Y., ... & Zhang, D. (2021). CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis. Aging (Albany NY), 13(9), 12780.

Li, Y., Su, G., Zhong, Y., Xiong, Z., Huang, T., Quan, J., ... & Lai, T. (2021). HB-EGF-induced IL-8 secretion from airway epithelium leads to lung fibroblast proliferation and migration. BMC Pulmonary Medicine, 21(1), 1-12.

Rao, L., Giannico, D., Leone, P., Solimando, A. G., Maiorano, E., Caporusso, C., ... & Racanelli, V. (2020). HB-EGF–EGFR signaling in bone marrow endothelial cells mediates angiogenesis associated with multiple myeloma. Cancers, 12(1), 173.

Fu, E., Liu, T., Yu, S., Chen, X., Song, L., Lou, H., ... & Wei, L. (2020). M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB‑EGF. Oncology Reports, 44(2), 698-710.

Eapen, M. S., Sharma, P., Thompson, I. E., Lu, W., Myers, S., Hansbro, P. M., & Sohal, S. S. (2019). Heparin-binding epidermal growth factor (HB-EGF) drives EMT in patients with COPD: implications for disease pathogenesis and novel therapies. Laboratory Investigation, 99(2), 150-157.

Wen, H. J., Gao, S., Wang, Y., Ray, M., Magnuson, M. A., Wright, C. V., ... & Crawford, H. C. (2019). Myeloid cell-derived HB-EGF drives tissue recovery after pancreatitis. Cellular and Molecular Gastroenterology and Hepatology, 8(2), 173-192.

Yang, C. C., & Chang, K. W. (2018). Eicosanoids and HB-EGF/EGFR in cancer. Cancer and Metastasis Reviews, 37, 385-395.

Wang, H. U. I., Shi, G., Li, M., Fan, H., Ma, H., & Sheng, L. I. (2018). Correlation of IL-1 and HB-EGF with endometrial receptivity. Experimental and therapeutic medicine, 16(6), 5130-5136.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant HBEGF protein?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-HBEGF Recombinant Antibody (1A4) (CAT#: CBMAB-A3753-LY)

Mouse Anti-HBEGF (AA 20-208) Recombinant Antibody (CBFYH-0750) (CAT#: CBMAB-H1653-FY)

Mouse Anti-HBEGF Recombinant Antibody (CBFYH-0749) (CAT#: CBMAB-H1652-FY)

Mouse Anti-HBEGF (AA 63-148) Recombinant Antibody (CBFYH-0745) (CAT#: CBMAB-H1649-FY)

Human Anti-HBEGF Recombinant Antibody (CBFYH-0748) (CAT#: CBMAB-H1651-FY)

Mouse Anti-HBEGF Recombinant Antibody (CBFYH-0746) (CAT#: CBMAB-H1650-FY)

Mouse Anti-HBEGF Recombinant Antibody (CBFYH-0747) (CAT#: CBMAB-H0355-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry