Human Recombinant HCK, Active protein, GST Tag (V2LY-0526-LY4461)

Name: Human Recombinant HCK, Active protein, GST Tag
SKU: V2LY-0526-LY4461
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant HCK, Active protein, GST Tag consist of Amino Acid: 251-518 and predicts a molecular mass of 57 kDa.

Molecule Mass

57 kDa

Sequence

Amino Acid: 251-518

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

HCK Proto-Oncogene, Src Family Tyrosine Kinase

Function

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Biological Process

Cell adhesion Source: UniProtKB
Cell differentiation Source: GO_Central
Cytokine-mediated signaling pathway Source: UniProtKB
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
Inflammatory response Source: UniProtKB-KW
Innate immune response Source: GO_Central
Innate immune response-activating signal transduction Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Interferon-gamma-mediated signaling pathway Source: UniProtKB
Leukocyte degranulation Source: UniProtKB
Leukocyte migration involved in immune response Source: UniProtKB
Lipopolysaccharide-mediated signaling pathway Source: UniProtKB
Mesoderm development Source: ProtInc
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of inflammatory response to antigenic stimulus Source: Reactome
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of actin cytoskeleton reorganization Source: UniProtKB
Positive regulation of actin filament polymerization Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Protein phosphorylation Source: ProtInc
Regulation of cell shape Source: UniProtKB
Regulation of DNA-binding transcription factor activity Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Regulation of phagocytosis Source: UniProtKB
Regulation of podosome assembly Source: UniProtKB
Respiratory burst after phagocytosis Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Isoform 1: Cytosol; Podosome membrane; Lysosome; Membrane. Associated with specialized secretory lysosomes called azurophil granules. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated.
Isoform 2: Golgi apparatus; Cytoskeleton; Nucleus; Lysosome; Cell membrane; Caveola; Focal adhesion; Cytoplasmic vesicle. 20% of this isoform is associated with caveolae. Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3. Colocalizes with the actin cytoskeleton at focal adhesions.
Cytosol; Secretory vesicle

Involvement in disease

Aberrant activation of HCK by HIV-1 protein Nef enhances HIV-1 replication and contributes to HIV-1 pathogenicity.
Aberrant activation of HCK, e.g. by the BCR-ABL fusion protein, promotes cancer cell proliferation.

PTM

Phosphorylated on several tyrosine residues. Autophosphorylated. Becomes rapidly phosphorylated upon activation of the immunoglobulin receptors FCGR1A and FCGR2A. Phosphorylation by the BCR-ABL fusion protein mediates activation of HCK. Phosphorylation at Tyr-411 increases kinase activity. Phosphorylation at Tyr-522 inhibits kinase activity. Kinase activity is not required for phosphorylation at Tyr-522, suggesting that this site is a target of other kinases.9 Publications
Ubiquitinated by CBL, leading to its degradation via the proteasome.
Isoform 2 palmitoylation at position 2 requires prior myristoylation. Palmitoylation at position 3 is required for caveolar localization of isoform 2.

Premanand, A., & Reena Rajkumari, B. (2023). Bioinformatic analysis of gene expression data reveals Src family protein tyrosine kinases as key players in androgenetic alopecia. Frontiers in Medicine, 10, 1108358.

Portugal, C. C., Almeida, T. O., Socodato, R., & Relvas, J. B. (2022). Src family kinases (SFKs): critical regulators of microglial homeostatic functions and neurodegeneration in Parkinson's and Alzheimer's diseases. The FEBS Journal, 289(24), 7760-7775.

Pond, M. P., Eells, R., Treece, B. W., Heinrich, F., Lösche, M., & Roux, B. (2020). Membrane anchoring of Hck kinase via the intrinsically disordered SH4-U and length scale associated with subcellular localization. Journal of molecular biology, 432(9), 2985-2997.

Sirvent, A., Mevizou, R., Naim, D., Lafitte, M., & Roche, S. (2020). Src family tyrosine kinases in intestinal homeostasis, regeneration and tumorigenesis. Cancers, 12(8), 2014.

Patel, R. K., Weir, M. C., Shen, K., Snyder, D., Cooper, V. S., & Smithgall, T. E. (2019). Expression of myeloid Src-family kinases is associated with poor prognosis in AML and influences Flt3-ITD kinase inhibitor acquired resistance. PLoS One, 14(12), e0225887.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

HCK Matched Antibody Pair (528) (CAT#: APMAB-528LY)

Antibody Pairs

HCK Matched Antibody Pair (528) (CAT#: APMAB-528LY)

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Isotype control

For research use only. Not intended for any clinical use.

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