Human Recombinant HJV protein, His Tag (V2LY-0526-LY4481)

Name: Human Recombinant HJV protein, His Tag
SKU: V2LY-0526-LY4481
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant HJV protein, His Tag consist of Amino Acid: 1-399 and predicts a molecular mass of 40 kDa.

Molecule Mass

40 kDa

Sequence

Amino Acid: 1-399

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

hemojuvelin BMP co-receptor

Function

Acts as a bone morphogenetic protein (BMP) coreceptor (PubMed:18976966).

Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis (PubMed:18976966).

Biological Process

Activin receptor signaling pathway Source: BHF-UCL
BMP signaling pathway Source: BHF-UCL
Cellular iron ion homeostasis Source: BHF-UCL
Cellular response to BMP stimulus Source: BHF-UCL
Iron ion homeostasis Source: MGI
Negative regulation of BMP signaling pathway Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Protein autoprocessing Source: BHF-UCL

Cellular Location

Cell membrane. Also released in the extracellular space.

Involvement in disease

Hemochromatosis 2A (HFE2A):
A juvenile form of hemochromatosis, a disorder of iron metabolism with excess deposition of iron in a variety of organs leading to their failure, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of juvenile hemochromatosis at presentation are hypogonadism and cardiomyopathy.

PTM

Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds (PubMed:25156943). Also proteolytically processed by TMPRSS6, several fragments being released in the extracellular space; regulates HJV activity in BMP signaling and thefore iron homeostasis (PubMed:18976966, PubMed:25156943).

Novikov, N., Buch, A., Yang, H., Tuller, S., Andruk, M., Chan, K., ... & Savage, W. (2022). DISC-0974, an anti-hemojuvelin antibody, reduces hepcidin and mobilizes iron in healthy volunteers. Blood, 140(Supplement 1), 5339-5340.

Tippairote, T., Bjørklund, G., Peana, M., & Roytrakul, S. (2021). The proteomics study of compounded HFE/TF/TfR2/HJV genetic variations in a Thai family with iron overload, chronic anemia, and motor neuron disorder. Journal of Molecular Neuroscience, 71(3), 545-555.

Pettinato, M., Aghajan, M., Olivari, V., Dulja, A., Nai, A., Bordini, J., ... & Silvestri, L. (2019). Targeting the BMP Receptor Inhibitor FKBP12 to Upregulate Hepcidin in Wild-Type and Hemojuvelin-Hemochromatosis Models. Blood, 134, 955.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant HJV protein, His Tag (CAT#: V2LY-0526-LY1530)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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