Human Recombinant HK2 protein, His Tag (V2LY-0526-LY4555)

Name: Human Recombinant HK2 protein, His Tag
SKU: V2LY-0526-LY4555
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant HK2 protein, His Tag consist of Amino Acid: 1-917 and predicts a molecular mass of 103.84 kDa.

Molecule Mass

103.84 kDa

Verified

HPLC

Sequence

Amino Acid: 1-917

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Hexokinase 2

Function

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880).

Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880).

Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).

Biological Process

Apoptotic mitochondrial changes Source: MGI
Canonical glycolysis Source: Reactome
Cellular glucose homeostasis Source: GO_Central
Cellular response to leukemia inhibitory factor Source: Ensembl
Establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
Fructose 6-phosphate metabolic process Source: UniProtKB
Glucose 6-phosphate metabolic process Source: UniProtKB
Glycolytic process Source: GO_Central
Lactation Source: Ensembl
Maintenance of protein location in mitochondrion Source: ParkinsonsUK-UCL
Negative regulation of mitochondrial membrane permeability Source: Ensembl
Negative regulation of reactive oxygen species metabolic process Source: Ensembl
Positive regulation of angiogenesis Source: BHF-UCL
Positive regulation of autophagy of mitochondrion in response to mitochondrial depolarization Source: ParkinsonsUK-UCL
Regulation of glucose import Source: Ensembl
Response to hypoxia Source: Ensembl
Response to ischemia Source: Ensembl

Cellular Location

Mitochondrion outer membrane; Cytosol. The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (PubMed:29298880). The interaction with the mitochondrial outer membrane via the mitochondrial-binding peptide (MBP) region promotes higher stability of the protein (PubMed:29298880). Release from the mitochondrial outer membrane into the cytosol induces permeability transition pore (PTP) opening and apoptosis (PubMed:18350175).

Wang, S., Zhuang, Y., Xu, J., Tong, Y., Li, X., & Dong, C. (2023). Advances in the Study of Hexokinase 2 (HK2) Inhibitors. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 23(7), 736-746.

Li, R., Mei, S., Ding, Q., Wang, Q., Yu, L., & Zi, F. (2022). A pan-cancer analysis of the role of hexokinase II (HK2) in human tumors. Scientific Reports, 12(1), 18807.

Ciscato, F., Ferrone, L., Masgras, I., Laquatra, C., & Rasola, A. (2021). Hexokinase 2 in cancer: a prima donna playing multiple characters. International journal of molecular sciences, 22(9), 4716.

Hinrichsen, F., Hamm, J., Westermann, M., Schröder, L., Shima, K., Mishra, N., ... & Sommer, F. (2021). Microbial regulation of hexokinase 2 links mitochondrial metabolism and cell death in colitis. Cell Metabolism, 33(12), 2355-2366.

Rabbani, N., & Thornalley, P. J. (2019). Hexokinase-2 glycolytic overload in diabetes and ischemia–reperfusion injury. Trends in Endocrinology & Metabolism, 30(7), 419-431.

Bao, F., Yang, K., Wu, C., Gao, S., Wang, P., Chen, L., & Li, H. (2018). New natural inhibitors of hexokinase 2 (HK2): Steroids from Ganoderma sinense. Fitoterapia, 125, 123-129.

Jiao, L., Zhang, H. L., Li, D. D., Yang, K. L., Tang, J., Li, X., ... & Zhu, X. F. (2018). Regulation of glycolytic metabolism by autophagy in liver cancer involves selective autophagic degradation of HK2 (hexokinase 2). Autophagy, 14(4), 671-684.

Bustamante, M. F., Oliveira, P. G., Garcia-Carbonell, R., Croft, A. P., Smith, J. M., Serrano, R. L., ... & Guma, M. (2018). Hexokinase 2 as a novel selective metabolic target for rheumatoid arthritis. Annals of the rheumatic diseases, 77(11), 1636-1643.

Yang, T., Ren, C., Qiao, P., Han, X., Wang, L., Lv, S., ... & Yu, Z. (2018). PIM2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and paclitaxel resistance in breast cancer. Oncogene, 37(45), 5997-6009.

Zhang, X. Y., Zhang, M., Cong, Q., Zhang, M. X., Zhang, M. Y., Lu, Y. Y., & Xu, C. J. (2018). Hexokinase 2 confers resistance to cisplatin in ovarian cancer cells by enhancing cisplatin-induced autophagy. The international journal of biochemistry & cell biology, 95, 9-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Recombinant HK2 protein, His Tag (CAT#: V2LY-0526-LY8401)

Pig HK2 ELISA Kit (CAT#: V2LY-0626-LY1038)

Rat Hk2 ELISA Kit (CAT#: V2LY-0626-LY2369)

Human HK2 ELISA Kit (CAT#: V2LY-0626-LY5607)

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Pig HK2 ELISA Kit (CAT#: V2LY-0626-LY1038)

Mouse Anti-HK2 Recombinant Antibody (CBFYH-1228) (CAT#: CBMAB-H2201-FY)

Rabbit Anti-HK2 Recombinant Antibody (H.738.7) (CAT#: CBMAB-H2198-FY)

Mouse Anti-HK2 Monoclonal Antibody (CBFYH-3091) (CAT#: CBMAB-H2195-FY)

Rabbit Anti-HK2 (AA 325-444) Recombinant Antibody (2H8L6) (CAT#: CBMAB-H2196-FY)

Human HK2 ELISA Kit (CAT#: V2LY-0626-LY5607)

Mouse Anti-HK2 Recombinant Antibody (CBFYH-1232) (CAT#: CBMAB-H0795-FY)

Mouse Anti-HK2 Recombinant Antibody (CBT2955) (CAT#: V2LY-0625-LY2446)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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