Human Recombinant ICAM3 protein, His Tag (V2LY-0526-LY4629)

Name: Human Recombinant ICAM3 protein, His Tag
SKU: V2LY-0526-LY4629
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant ICAM3 protein, His Tag consist of Amino Acid: 1-485 and predicts a molecular mass of 50.8 kDa.

Molecule Mass

50.8 kDa

Sequence

Amino Acid: 1-485

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>92% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glycine

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Intercellular Adhesion Molecule 3

Function

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173).

ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590).

Biological Process

Cell adhesion Source: GO_Central
Cell-cell adhesion Source: InterPro
Phagocytosis Source: UniProtKB-KW

Cellular Location

Membrane

Topology

Extracellular: 30-485
Helical: 486-510
Cytoplasmic: 511-547

PTM

Upon stimulation by a physiologic stimuli becomes rapidly and transiently phosphorylated on serine residues.
N-glycosylated; glycans consist of a mixture of tri- and tetra-antennary complex-type chains and high-mannose chains.

Li, L., Yang, C., Aruna, Zhou, Q., Jiang, X., Du, W., ... & Shen, W. (2022). Functional evaluation of various ICAM3 transcript variants in diffuse large B-Cell lymphoma. Leukemia & Lymphoma, 63(12), 2869-2878.

Zicheng, H., Xiao, Y., Rongzhong, H., Yongyong, L., Haitao, R., & Tingting, S. (2021). Association of Circulating ICAM3 Concentrations with Severity and Short-term Outcomes of Acute Ischemic Stroke. Neurotoxicity Research, 39(4), 1293-1299.

Chen, W. Y., Wu, S. Y., Lin, T. C., Lin, S. L., & Wu-Hsieh, B. A. (2019). Human dendritic cell-specific ICAM-3-grabbing non-integrin downstream signaling alleviates renal fibrosis via Raf-1 activation in systemic candidiasis. Cellular & Molecular Immunology, 16(3), 288-301.

Demiryürek, S., Saracaloglu, A., Kimyon, S., Mete, A., Eronat, O., Temiz, E., ... & Demiryürek, A. T. (2019). Increased Expressions of ICAM-2 and ICAM-3 in Pterygium. Current eye research, 44(6), 645-650.

Cruz, L. J., Tacken, P. J., van der Schoot, J. M., Rueda, F., Torensma, R., & Figdor, C. G. (2019). ICAM3-Fc outperforms receptor-specific antibodies targeted nanoparticles to dendritic cells for cross-presentation. Molecules, 24(9), 1825.

Velasquez, L. N., Stüve, P., Gentilini, M. V., Swallow, M., Bartel, J., Lycke, N. Y., ... & Berod, L. (2018). Targeting Mycobacterium tuberculosis antigens to dendritic cells via the DC-specific-ICAM3-grabbing-nonintegrin receptor induces strong T-helper 1 immune responses. Frontiers in immunology, 9, 471.

Chéneau, C., Coulon, F., Porkolab, V., Fieschi, F., Laurant, S., Razanajaona-Doll, D., ... & Halary, F. (2018). Fine mapping the interaction between dendritic cell-specific intercellular adhesion molecule (ICAM)-3-grabbing nonintegrin and the cytomegalovirus envelope glycoprotein B. The Journal of Infectious Diseases, 218(3), 490-503.

Shen, W., Xie, J., Zhao, S., Du, R., Luo, X., He, H., ... & Luo, Y. (2018). ICAM3 mediates inflammatory signaling to promote cancer cell stemness. Cancer letters, 422, 29-43.

Shen, W., Zhang, X., Du, R., Fan, Y., Luo, D., Bao, Y., ... & Zhao, S. (2018). ICAM3 mediates tumor metastasis via a LFA-1-ICAM3-ERM dependent manner. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(8), 2566-2578.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human ICAM3 ELISA Kit (CAT#: V2LY-0626-LY638)

Human Recombinant ICAM3 protein, His & hFc Tag (CAT#: V2LY-0526-LY4628)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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