Human Recombinant IDS protein, His Tag (V2LY-0526-LY4642)

Name: Human Recombinant IDS protein, His Tag
SKU: V2LY-0526-LY4642
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant IDS protein, His Tag consist of Amino Acid: 1-550 and predicts a molecular mass of 61 kDa.

Molecule Mass

61 kDa

Sequence

Amino Acid: 1-550

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>87% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Sodium phosphate, NaCl, Imidazole, PMSF, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Iduronate 2-Sulfatase

Function

Lysosomal enzyme involved in the degradation pathway of dermatan sulfate and heparan sulfate.

Biological Process

Glycosaminoglycan catabolic process Source: UniProtKB

Cellular Location

Lysosome

Involvement in disease

Mucopolysaccharidosis 2 (MPS2):
An X-linked lysosomal storage disease characterized by intracellular accumulation of heparan sulfate and dermatan sulfate and their excretion in urine. Most children with MPS2 have a severe form with early somatic abnormalities including skeletal deformities, hepatosplenomegaly, and progressive cardiopulmonary deterioration. A prominent feature is neurological damage that presents as developmental delay and hyperactivity but progresses to mental retardation and dementia. They die before 15 years of age, usually as a result of obstructive airway disease or cardiac failure. In contrast, those with a mild form of MPS2 may survive into adulthood, with attenuated somatic complications and often without mental retardation.

PTM

Synthesized as a 75-kDa precursor form in the endoplasmic reticulum (ER), and then processed by proteolytic cleavage through various intermediates to yield a 55-kDa mature form, with the release of an 18 kDa polypeptide.
The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

Badenetti, L., Manzoli, R., Trevisan, M., D’Avanzo, F., Tomanin, R., & Moro, E. (2023). A novel CRISPR/Cas9-based iduronate-2-sulfatase (IDS) knockout human neuronal cell line reveals earliest pathological changes. Scientific Reports, 13(1), 10289.

Mashima, R., Ohira, M., Okuyama, T., Onodera, M., & Takada, S. (2023). A novel mucopolysaccharidosis type II mouse model with an iduronate-2-sulfatase-P88L mutation. Scientific Reports, 13(1), 7865.

Gusarova, V. D., Smolov, M. A., Lyagoskin, I. V., Degterev, M. B., Rechetnik, E. V., Rodionov, A. V., ... & Shukurov, R. R. (2023). Characterization of a HIR-Fab-IDS, Novel Iduronate 2-Sulfatase Fusion Protein for the Treatment of Neuropathic Mucopolysaccharidosis Type II (Hunter Syndrome). BioDrugs, 37(3), 375-395.

Arguello, A., Meisner, R., Thomsen, E. R., Nguyen, H. N., Ravi, R., Simms, J., ... & Sanchez, P. E. (2021). Iduronate-2-sulfatase transport vehicle rescues behavioral and skeletal phenotypes in a mouse model of Hunter syndrome. JCI insight, 6(19).

Giugliani, R., Martins, A. M., So, S., Yamamoto, T., Yamaoka, M., Ikeda, T., ... & Sato, Y. (2021). Iduronate-2-sulfatase fused with anti-hTfR antibody, pabinafusp alfa, for MPS-II: A phase 2 trial in Brazil. Molecular Therapy, 29(7), 2378-2386.

Ohira, M., Kikuchi, E., Mizuta, S., Yoshida, N., Onodera, M., Nakanishi, M., ... & Mashima, R. (2021). Production of therapeutic iduronate‐2‐sulfatase enzyme with a novel single‐stranded RNA virus vector. Genes to Cells, 26(11), 891-904.

Okuyama, T., Eto, Y., Sakai, N., Minami, K., Yamamoto, T., Sonoda, H., ... & Sato, Y. (2019). Iduronate-2-sulfatase with anti-human transferrin receptor antibody for neuropathic mucopolysaccharidosis II: a phase 1/2 trial. Molecular Therapy, 27(2), 456-464.

Pimentel, N., Rodríguez‐Lopez, A., Díaz, S., Losada, J. C., Díaz‐Rincón, D. J., Cardona, C., ... & Barrera‐Avellaneda, L. A. (2018). Production and characterization of a human lysosomal recombinant iduronate‐2‐sulfatase produced in Pichia pastoris. Biotechnology and applied biochemistry, 65(5), 655-664.

Osaki, Y., Saito, A., Kanemoto, S., Kaneko, M., Matsuhisa, K., Asada, R., ... & Imaizumi, K. (2018). Shutdown of ER-associated degradation pathway rescues functions of mutant iduronate 2-sulfatase linked to mucopolysaccharidosis type II. Cell Death & Disease, 9(8), 808.

Hoshina, H., Shimada, Y., Higuchi, T., Kobayashi, H., Ida, H., & Ohashi, T. (2018). Chaperone effect of sulfated disaccharide from heparin on mutant iduronate-2-sulfatase in mucopolysaccharidosis type II. Molecular genetics and metabolism, 123(2), 118-122.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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