Human Recombinant IL12RB1 protein, ECD, His Tag (V2LY-0526-LY4734)

Name: Human Recombinant IL12RB1 protein, ECD, His Tag
SKU: V2LY-0526-LY4734
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant IL12RB1 protein, ECD, His Tag consist of Amino Acid: 1-540 and predicts a molecular mass of 58.5 kDa.

Molecule Mass

58.5 kDa

Protein Domain

ECD

Sequence

Amino Acid: 1-540

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>94% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS, Glycerol, SKL

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Interleukin 12 Receptor Subunit Beta 1

Function

Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

Biological Process

Cellular response to interferon-gamma Source: BHF-UCL
Cytokine-mediated signaling pathway Source: GO_Central
Interleukin-12-mediated signaling pathway Source: GOC
Interleukin-23-mediated signaling pathway Source: GOC
Positive regulation of activated T cell proliferation Source: BHF-UCL
Positive regulation of defense response to virus by host Source: BHF-UCL
Positive regulation of interferon-gamma production Source: BHF-UCL
Positive regulation of memory T cell differentiation Source: BHF-UCL
Positive regulation of natural killer cell proliferation Source: ComplexPortal
Positive regulation of NK T cell proliferation Source: ComplexPortal
Positive regulation of receptor signaling pathway via JAK-STAT Source: ComplexPortal
Positive regulation of T cell mediated cytotoxicity Source: BHF-UCL
Positive regulation of T cell proliferation Source: ComplexPortal
Positive regulation of T-helper 17 cell lineage commitment Source: BHF-UCL
Positive regulation of T-helper 17 type immune response Source: BHF-UCL
Positive regulation of T-helper 1 type immune response Source: BHF-UCL
Signal transduction Source: BHF-UCL

Cellular Location

Membrane

Involvement in disease

Immunodeficiency 30 (IMD30):
A form of Mendelian susceptibility to mycobacterial disease, a rare condition caused by impairment of interferon-gamma mediated immunity. It is characterized by predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine, environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. Clinical outcome severity depends on the degree of impairment of interferon-gamma mediated immunity. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. IMD30 has low penetrance, and affected individuals have relatively mild disease and good prognosis. BCG disease and salmonellosis are the most frequent infections in IMD30 patients.

Topology

Extracellular: 24-545
Helical: 546-570
Cytoplasmic: 571-662

Abdelmajeed, O., Ali, M. M. D., Erwa, N. H., Mustafa, A., Ahmed, Y. A., Ahmed, R. H. A., ... & Algam, S. (2023). Autosomal recessive IL12RB1 mutation: A case report of a Sudanese child and his father. Frontiers in Immunology, 14, 1135824.

Wang, X., Jia, L., Liu, Y., Wang, J., Qiu, C., Li, T., ... & Wan, Y. (2022). Immune Correlates of Disseminated BCG Infection in IL12RB1-Deficient Mice. Vaccines, 10(7), 1147.

Mejia, O. R., Claeys, T. A., Williams, A., Zafar, A., & Robinson, R. T. (2022). IL12RB1 allele bias in human TH cells is regulated by functional SNPs in its 3′ UTR. Cytokine, 158, 155993.

Sharifinejad, N., Mahdaviani, S. A., Jamee, M., Daneshmandi, Z., Moniri, A., Marjani, M., ... & Velayati, A. A. (2021). Leukocytoclastic vasculitis in patients with IL12B or IL12RB1 deficiency: case report and review of the literature. Pediatric Rheumatology, 19(1), 1-8.

Liu, M., Lu, B., Zeng, P., Huang, B., Xu, Y., Liang, H., ... & Zhang, Y. (2020). Compound Heterozygous Mutations of IL12RB1 in a Patient with Selective Defects in Th17 Differentiation. Journal of Clinical Immunology, 40, 647-652.

Zhou, X., Jia, W., Ni, Z., Wang, A., Liu, Z., Hou, M., ... & Zhang, X. (2019). Three novel compound heterozygous IL12RB1 mutations in Chinese patients with Mendelian susceptibility to mycobacterial disease. Plos one, 14(4), e0215648.

Reeme, A. E., Claeys, T. A., Aggarwal, P., Turner, A. J., Routes, J. M., Broeckel, U., & Robinson, R. T. (2019). Human IL12RB1 expression is allele-biased and produces a novel IL12 response regulator. Genes & Immunity, 20(3), 181-197.

Al-Kzayer, L. A. F. Y., Yassin, A. K., Salih, K. H., Shigemura, T., Sano, K., Al-Simaani, R. B. Y., ... & Okuno, Y. (2019). A Syrian refugee in Iraq diagnosed as a case of IL12RB1 deficiency in Japan using dried blood spots. Frontiers in immunology, 10, 58.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant IL12RB1 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY4733)

Human Recombinant IL12RB1 protein, ECD, His Tag-1 (CAT#: V2LY-0526-LY4735)

Cynomolgus Recombinant IL12RB1 protein, ECD, His Tag (CAT#: V2LY-0526-LY1547)

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Isotype control

For research use only. Not intended for any clinical use.

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