Human Recombinant IL21, GMP grade, Animal-Free protein (V2LY-0526-LY4834)

Name: Human Recombinant IL21, GMP grade, Animal-Free protein
SKU: V2LY-0526-LY4834
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Protein Construction

This product is Human Recombinant IL21, GMP grade, Animal-Free protein consist of Amino Acid: 30-162 and predicts a molecular mass of 15.6 kDa.

Molecule Mass

15.6 kDa

Verified

HPLC

Sequence

Amino Acid: 30-162

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile

Preservative

None

Storage

Samples are stable for up to thirty-six months from date of receipt at ≤ -20°C
Store it under sterile conditions at ≤-20°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

interleukin 21

Function

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity).
Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity).
May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (PubMed:11081504, PubMed:15178704).
During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity).

Biological Process

MaturationManual Assertion Based On ExperimentIDA:UniProtKB
Cellular response to virusISS:UniProtKB
Defense response to virusManual Assertion Based On ExperimentTAS:ARUK-UCL
Germinal center B cell differentiationISS:UniProtKB
Positive regulation of B cell proliferationManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cell population proliferationManual Assertion Based On ExperimentIDA:MGI
Positive regulation of cytokine productionManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of immunoglobulin productionISS:UniProtKB
Positive regulation of inflammatory response1 PublicationIC:BHF-UCL
Positive regulation of interferon-gamma production1 PublicationNAS:UniProtKB
Positive regulation of interleukin-17 productionManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIDA:CACAO
Positive regulation of T cell proliferationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of tissue remodeling1 PublicationIC:BHF-UCL
Positive regulation of tyrosine phosphorylation of STAT proteinManual Assertion Based On ExperimentIDA:MGI
Signal transduction1 PublicationNAS:UniProtKB
T follicular helper cell differentiationISS:UniProtKB
Tyrosine phosphorylation of STAT proteinManual Assertion Based On ExperimentIDA:CACAO

Cellular Location

Secreted

Involvement in disease

Immunodeficiency, common variable, 11 (CVID11):
A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Nguyen, R., Zhang, X., Sun, M., Abbas, S., Seibert, C., Kelly, M. C., ... & Thiele, C. J. (2022). Anti-Gd2 antibodies conjugated to IL15 and IL21 mediate potent antitumor cytotoxicity against neuroblastoma. Clinical Cancer Research, 28(17), 3785-3796.

Loucif, H., Dagenais-Lussier, X., Avizonis, D., Choinière, L., Beji, C., Cassin, L., ... & van Grevenynghe, J. (2022). Autophagy-dependent glutaminolysis drives superior IL21 production in HIV-1-specific CD4 T cells. Autophagy, 18(6), 1256-1273.

Hao, H., Nakayamada, S., Ohkubo, N., Yamagata, K., Zhang, M., Shan, Y., ... & Tanaka, Y. (2021). Involvement of lncRNA IL21-AS1 in interleukin-2 and T follicular regulatory cell activation in systemic lupus erythematosus. Arthritis Research & Therapy, 23(1), 302.

Wu, S., Sun, R., Tan, B., Chen, B., Zhou, W., Gao, D. S., ... & Lu, B. (2021). The half-life-extended IL21 can be combined with multiple checkpoint inhibitors for tumor immunotherapy. Frontiers in Cell and Developmental Biology, 9, 779865.

Gonzalez-Junca, A., Liu, F. D., Nagaraja, A. S., Mullenix, A., Lee, C. T., Gordley, R. M., ... & Lee, G. (2021). SENTI-101, a preparation of mesenchymal stromal cells engineered to express IL12 and IL21, induces localized and durable antitumor immunity in preclinical models of peritoneal solid tumors. Molecular Cancer Therapeutics, 20(9), 1508-1520.

Wang, J., Hasan, F., Frey, A. C., Li, H. S., Park, J., Pan, K., ... & Yee, C. (2020). Histone deacetylase inhibitors and IL21 cooperate to reprogram human effector CD8+ T cells to memory T cells. Cancer immunology research, 8(6), 794-805.

Batra, S. A., Rathi, P., Guo, L., Courtney, A. N., Fleurence, J., Balzeau, J., ... & Heczey, A. (2020). Glypican-3–specific CAR T cells coexpressing IL15 and IL21 have superior expansion and antitumor activity against hepatocellular carcinoma. Cancer immunology research, 8(3), 309-320.

Ciszewski, C., Discepolo, V., Pacis, A., Doerr, N., Tastet, O., Mayassi, T., ... & Jabri, B. (2020). Identification of a γc receptor antagonist that prevents reprogramming of human tissue-resident cytotoxic T cells by IL15 and IL21. Gastroenterology, 158(3), 625-637.

Desmots, F., Roussel, M., Pangault, C., Llamas-Gutierrez, F., Pastoret, C., Guiheneuf, E., ... & Fest, T. (2019). Pan-HDAC inhibitors restore PRDM1 response to IL21 in CREBBP-mutated follicular lymphoma. Clinical Cancer Research, 25(2), 735-746.

Seo, H., Kim, B. S., Bae, E. A., Min, B. S., Han, Y. D., Shin, S. J., & Kang, C. Y. (2018). IL21 therapy combined with PD-1 and Tim-3 blockade provides enhanced NK cell antitumor activity against MHC class I–deficient tumors. Cancer immunology research, 6(6), 685-695.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Canine Recombinant IL-21 protein (CAT#: V2LY-0526-LY1279)

Human Recombinant IL21 protein (CAT#: V2LY-0526-LY4836)

Mouse Recombinant IL21 protein (CAT#: V2LY-0526-LY8515)

Rat Recombinant IL21 protein (CAT#: V2LY-0526-LY9458)

Rat Recombinant IL21 protein, His Tag (CAT#: V2LY-0526-LY9459)

Rhesus Recombinant IL21 protein (CAT#: V2LY-0526-LY9747)

Human Recombinant IL21, GMP grade, DMF Filed protein (CAT#: V2LY-0526-LY4835)

Related Products

Canine Recombinant IL-21 protein (CAT#: V2LY-0526-LY1279)

Human Recombinant IL21, GMP grade, DMF Filed protein (CAT#: V2LY-0526-LY4835)

Rat Recombinant IL21 protein (CAT#: V2LY-0526-LY9458)

Rabbit Anti-IL21 Recombinant Antibody (101) (CAT#: CBMAB-I0490-YY)

Mouse Anti-IL21 Recombinant Antibody (CBYY-I1719) (CAT#: CBMAB-I2889-YY)

Rat Anti-IL21 Recombinant Antibody (CBYY-I0177) (CAT#: CBMAB-I0491-YY)

Rhesus Recombinant IL21 protein (CAT#: V2LY-0526-LY9747)

Human Recombinant IL21 protein (CAT#: V2LY-0526-LY4836)

Mouse Anti-IL21 Recombinant Antibody (CBYY-I0180) (CAT#: CBMAB-I0495-YY)

Rat Anti-IL21 Recombinant Antibody (CBYY-I0328) (CAT#: CBMAB-I0864-YY)

Isotype control

For research use only. Not intended for any clinical use.

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