Human Recombinant INCENP protein, His Tag (V2LY-0526-LY4994)

Name: Human Recombinant INCENP protein, His Tag
SKU: V2LY-0526-LY4994
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant INCENP protein, His Tag consist of Amino Acid: 783-end and predicts a molecular mass of 19 kDa.

Molecule Mass

19 kDa

Sequence

Amino Acid: 783-end

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Inner Centromere Protein

Function

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Acts as a scaffold regulating CPC localization and activity. The C-terminus associates with AURKB or AURKC, the N-terminus associated with BIRC5/survivin and CDCA8/borealin tethers the CPC to the inner centromere, and the microtubule binding activity within the central SAH domain directs AURKB/C toward substrates near microtubules (PubMed:15316025, PubMed:12925766, PubMed:27332895).
The flexibility of the SAH domain is proposed to allow AURKB/C to follow substrates on dynamic microtubules while ensuring CPC docking to static chromatin (By similarity).
Activates AURKB and AURKC (PubMed:27332895).
Required for localization of CBX5 to mitotic centromeres (PubMed:21346195).
Controls the kinetochore localization of BUB1 (PubMed:16760428).

Biological Process

Chromosome segregationManual Assertion Based On ExperimentIMP:UniProtKB
Histone phosphorylationManual Assertion Based On ExperimentIBA:GO_Central
Meiotic spindle midzone assemblyManual Assertion Based On ExperimentIBA:GO_Central
Metaphase plate congressionManual Assertion Based On ExperimentIBA:GO_Central
Mitotic cytokinesisManual Assertion Based On ExperimentIMP:UniProtKB
Mitotic sister chromatid segregationIEA:InterPro
Positive regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIBA:GO_Central
Regulation of mitotic cytokinesisIEA:InterPro

Cellular Location

Nucleus; Cytoplasm, cytoskeleton, spindle; Midbody; Chromosome, centromere, kinetochore. Colocalized at synaptonemal complex central element from zygotene up to late pachytene when it begins to relocalize to heterochromatic chromocenters. Colocalizes with AURKB at a connecting strand traversing the centromere region and joining sister kinetochores, in metaphase II centromeres. This strand disappears at the metaphase II/anaphase II transition and relocalizes to the spindle midzone (By similarity).
Colocalizes with AURKB at mitotic chromosomes (PubMed:11453556).
Localizes to inner kinetochore (PubMed:16760428).
Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis (PubMed:15316025).
Cocalizes to the equatorial cell cortex at anaphase (PubMed:11453556).

PTM

Phosphorylation by AURKB or AURKC at its C-terminal part is important for AURKB or AURKC activation by INCENP.

Redouane, S., Charoute, H., Harmak, H., Malki, A., Barakat, A., & Rouba, H. (2022). Computational study of the potential impact of AURKC missense SNPs on AURKC-INCENP interaction and their correlation to macrozoospermia. Journal of Biomolecular Structure and Dynamics, 1-20.

Shukla, S., Bhattacharya, A., Agarwal, P., Malik, N., Duraisamy, K., Sri Rangan, N., ... & Kumar, A. (2022). Intrinsic disorder in CENP-ACse4 tail and its chaperone facilitates synergistic association for kinetochore stabilization. bioRxiv, 2022-08.

Petsalaki, E., & Zachos, G. (2021). An ATM-CHK2-INCENP pathway prevents chromatin breakage by regulating the abscission checkpoint. Molecular & Cellular Oncology, 8(2), 1877999.

Zhang, C., Xie, L., Fu, Y., Yang, J., & Cui, Y. (2020). lncRNA MIAT promotes esophageal squamous cell carcinoma progression by regulating miR‐1301‐3p/INCENP axis and interacting with SOX2. Journal of cellular physiology, 235(11), 7933-7944.

Petsalaki, E., & Zachos, G. (2020). An ATM–Chk2–INCENP pathway activates the abscission checkpoint. Journal of Cell Biology, 220(2), e202008029.

García-Rodríguez, L. J., Kasciukovic, T., Denninger, V., & Tanaka, T. U. (2019). Aurora B-INCENP localization at centromeres/inner kinetochores is required for chromosome bi-orientation in budding yeast. Current Biology, 29(9), 1536-1544.

Abdul Azeez, K. R., Chatterjee, S., Yu, C., Golub, T. R., Sobott, F., & Elkins, J. M. (2019). Structural mechanism of synergistic activation of Aurora kinase B/C by phosphorylated INCENP. Nature communications, 10(1), 3166.

Sun, M., Veschi, V., Bagchi, S., Xu, M., Mendoza, A., Liu, Z., & Thiele, C. J. (2019). Targeting the chromosomal passenger complex subunit INCENP induces polyploidization, apoptosis, and senescence in neuroblastoma. Cancer research, 79(19), 4937-4950.

Papini, D., Fant, X., Ogawa, H., Desban, N., Samejima, K., Feizbakhsh, O., ... & Ruchaud, S. (2019). Cell cycle-independent furrowing triggered by phosphomimetic mutations of the INCENP STD motif requires Plk1. Journal of cell science, 132(21), jcs234401.

Feng, H., Raasholm, M., Moosmann, A., Campsteijn, C., & Thompson, E. M. (2019). Switching of INCENP paralogs controls transitions in mitotic chromosomal passenger complex functions. Cell Cycle, 18(17), 2006-2025.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
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Isotype control

For research use only. Not intended for any clinical use.

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