Human Recombinant JCHAIN protein, His Tag (V2LY-0526-LY5089)

Name: Human Recombinant JCHAIN protein, His Tag
SKU: V2LY-0526-LY5089
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant JCHAIN protein, His Tag consist of Amino Acid: 23-159 and predicts a molecular mass of 17 kDa.

Molecule Mass

17 kDa

Sequence

Amino Acid: 23-159

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile MES, NaCl, Glycerol, Trehalose

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

PREDICTED: similar to immunoglobulin J chain

Function

Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to secretory component.

Biological Process

Adaptive immune responseManual Assertion Based On ExperimentIDA:UniProtKB
Antibacterial humoral responseManual Assertion Based On ExperimentIDA:UniProtKB
Glomerular filtrationManual Assertion Based On ExperimentIMP:UniProtKB
Humoral immune responseManual Assertion Based On ExperimentIBA:GO_Central
Immune response1 PublicationNAS:UniProtKB
Innate immune responseManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of respiratory burstManual Assertion Based On ExperimentIDA:UniProtKB
Protein-containing complex assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Retina homeostasisManual Assertion Based On ExperimentHEP:UniProtKB

Cellular Location

Secreted

Matsumoto, M. L. (2022). Molecular mechanisms of multimeric assembly of IgM and IgA. Annual Review of Immunology, 40, 221-247.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human JCHAIN ELISA Kit (CAT#: V2LY-0626-LY3812)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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