Human Recombinant KIR2DL4 protein (V2LY-0526-LY5170)

Name: Human Recombinant KIR2DL4 protein
SKU: V2LY-0526-LY5170
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

CHO Stable Cells

Protein Species

Human

Protein Construction

This product is Human Recombinant KIR2DL4 protein consist of Amino Acid: 1-242 and predicts a molecular mass of 25.1 kDa.

Molecule Mass

25.1 kDa

Sequence

Amino Acid: 1-242

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Killer Cell Immunoglobulin Like Receptor, Two Ig Domains And Long Cytoplasmic Tail 4

Function

Receptor for non-classical major histocompatibility class Ib HLA-G molecules. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M). In decidual NK cells, binds peptide-bound HLA-G-B2M complex and triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy (PubMed:23184984, PubMed:29262349, PubMed:16366734).
May play a role in balancing tolerance and antiviral-immunity at maternal-fetal interface by keeping in check the effector functions of NK, CD8+ T cells and B cells (PubMed:10190900, PubMed:16366734).
Upon interaction with peptide-bound HLA-G-B2M, initiates signaling from the endosomal compartment leading to downstream activation of PRKDC-XRCC5 and AKT1, and ultimately triggering NF-kappa-B-dependent proinflammatory response (PubMed:20179272).

Biological Process

Cellular defense responseManual Assertion Based On ExperimentTAS:ProtInc
Negative regulation of natural killer cell mediated cytotoxicityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of cellular senescenceManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of natural killer cell cytokine productionManual Assertion Based On ExperimentIDA:UniProtKB
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Cell membrane; Early endosome membrane

Topology

Extracellular: 22-242
Helical: 243-263
Cytoplasmic: 264-377

Kilic, N., Dastouri, M., Kandemir, I., & Yilmaz, E. (2023). The effects of KIR2DL4 stimulated NK-92 cells on the apoptotic pathways of HER2+/HER-breast cancer cells. Medical Oncology, 40(5), 139.

Feng, Z., Huang, P., Zhang, J., Xia, X., Zhang, A. M., Zeng, T., ... & Yue, M. (2023). KIR2DL4/HLA‐G polymorphisms were associated with HCV infection susceptibility among Chinese high‐risk population. Journal of Medical Virology, 95(3), e28645.

Mao, R., Ren, Z., Yang, F., Yang, P., & Zhang, T. (2022). Clinical significance and immune landscape of KIR2DL4 and the senescence‐based signature in cutaneous melanoma. Cancer Science, 113(11), 3947.

Zheng, G., Jia, L., & Yang, A. G. (2022). Roles of HLA-G/KIR2DL4 in breast cancer immune microenvironment. Frontiers in Immunology, 13, 791975.

Ding, X. F., Chen, J., Ma, H. L., Liang, Y., Wang, Y. F., Zhang, H. T., ... & Chen, G. (2022). KIR2DL4 promotes the proliferation of RCC cell associated with PI3K/Akt signaling activation. Life Sciences, 293, 120320.

Zheng, G., Guo, Z., Li, W., Xi, W., Zuo, B., Zhang, R., ... & Jia, L. (2021). Interaction between HLA-G and NK cell receptor KIR2DL4 orchestrates HER2-positive breast cancer resistance to trastuzumab. Signal Transduction and Targeted Therapy, 6(1), 236.

Bai, Y., Liang, J., Liu, W., Wang, F., & Li, C. (2020). Possible roles of HLA-G regulating immune cells in pregnancy and endometrial diseases via KIR2DL4. Journal of Reproductive Immunology, 142, 103176.

Bespalova, O., Bakleicheva, M., Ivashchenko, T., Tral, T., Tolibova, G., & Kogan, I. (2020). Expression of HLA-G and KIR2DL4 receptor in chorionic villous in missed abortion. Gynecological Endocrinology, 36(sup1), 43-47.

Banerjee, P. P., Pang, L., Soldan, S. S., Miah, S. M., Eisenberg, A., Maru, S., ... & Orange, J. S. (2019). KIR2DL4-HLAG interaction at human NK cell-oligodendrocyte interfaces regulates IFN-γ-mediated effects. Molecular immunology, 115, 39-55.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant KIR2DL4 protein, hFc Tag (CAT#: V2LY-0526-LY5171)

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Isotype control

For research use only. Not intended for any clinical use.

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