Human Recombinant KIR3DL3 protein, ECD, His Tag (V2LY-0526-LY5176)

Name: Human Recombinant KIR3DL3 protein, ECD, His Tag
SKU: V2LY-0526-LY5176
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant KIR3DL3 protein, ECD, His Tag consist of Amino Acid: 1-322 and predicts a molecular mass of 33.7 kDa.

Molecule Mass

33.7 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-322

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥85% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Killer Cell Immunoglobulin Like Receptor, Three Ig Domains And Long Cytoplasmic Tail 3

Function

Receptor on natural killer cells. May inhibit the activity of NK cells thus preventing cell lysis.

Cellular Location

Cell membrane

Topology

Extracellular: 26-322
Helical: 323-343
Cytoplasmic: 344-410

Palmer, W. H., Leaton, L. A., Campos Codo, A., Crute, B., Roest, J., Zhu, S., ... & Norman, P. J. (2023). Polymorphic KIR3DL3 expression modulates tissue-resident and innate-like T cells. Science Immunology, 8(84), eade5343.

Li, Y., Lv, C., Yu, Y., Wu, B., Zhang, Y., Lang, Q., ... & Tian, Y. (2023). KIR3DL3-HHLA2 and TMIGD2-HHLA2 pathways: The dual role of HHLA2 in immune responses and its potential therapeutic approach for cancer immunotherapy. Journal of advanced research, 47, 137-150.

Lamb, M., Wei, Y., Ren, X., O'Connor, R., Dulak, A., Rausch, M., ... & Zang, X. (2022). 489 NPX267, a first-in-class monoclonal antibody targeting KIR3DL3, blocks HHLA2-mediated immunosuppression and potentiates T and NK cell-mediated antitumor immunity.

Wei, Y., Ren, X., Galbo Jr, P. M., Moerdler, S., Wang, H., Sica, R. A., ... & Zang, X. (2021). KIR3DL3-HHLA2 is a human immunosuppressive pathway and a therapeutic target. Science immunology, 6(61), eabf9792.

Bhatt, R. S., Berjis, A., Konge, J. C., Mahoney, K. M., Klee, A. N., Freeman, S. S., ... & Freeman, G. J. (2021). KIR3DL3 is an inhibitory receptor for HHLA2 that mediates an alternative immunoinhibitory pathway to PD1. Cancer immunology research, 9(2), 156-169.

Campbell, K. S. (2021). Mystery checkpoint revealed: KIR3DL3 finally found a ligand in HHLA2. Cancer Immunology Research, 9(2), 128-128.

Nutalai, R., Gaudieri, S., Jumnainsong, A., & Leelayuwat, C. (2019). Regulation of KIR3DL3 expression via mirna. Genes, 10(8), 603.

Leaton, L. A., Shortt, J., Kichula, K. M., Tao, S., Nemat-Gorgani, N., Mentzer, A. J., ... & Norman, P. J. (2019). Conservation, extensive heterozygosity, and convergence of signaling potential all indicate a critical role for KIR3DL3 in higher primates. Frontiers in Immunology, 10, 24.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant KIR3DL3 protein, His Tag (CAT#: V2LY-0526-LY1589)

Human Recombinant KIR3DL3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY5175)

Related Products

Cynomolgus Recombinant KIR3DL3 protein, His Tag (CAT#: V2LY-0526-LY1589)

Rabbit Anti-KIR3DL3 Recombinant Antibody (1136B) (CAT#: CBMAB-K0115-LY)

Human Recombinant KIR3DL3 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY5175)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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