Human Recombinant KLRB1 protein, hFc Tag (V2LY-0526-LY5194)

Name: Human Recombinant KLRB1 protein, hFc Tag
SKU: V2LY-0526-LY5194
Rating: 5 (1 reviews)

See all KLRB1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant KLRB1 protein, hFc Tag consist of Amino Acid: 67-225 and predicts a molecular mass of 45.14 kDa.

Molecule Mass

45.14 kDa

Verified

HPLC

Sequence

Amino Acid: 67-225

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Killer Cell Lectin Like Receptor B1

Function

Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells.

Biological Process

Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Membrane

Topology

Cytoplasmic: 1-44
Helical: 45-66
Extracellular: 67-225

PTM

N-glycosylated. Contains sialic acid residues.

Xu, N., Meng, X., Chu, H., Yang, Z., Jiao, Y., & Li, Y. (2023). The prognostic significance of KLRB1 and its further association with immune cells in breast cancer. PeerJ, 11, e15654.

Huang, G., Xiao, S., Jiang, Z., Zhou, X., Chen, L., Long, L., ... & Jiang, B. (2023). Machine learning immune-related gene based on KLRB1 model for predicting the prognosis and immune cell infiltration of breast cancer. Frontiers in Endocrinology, 14, 1185799.

Cheng, X., Cao, Y., Wang, X., Cheng, L., Liu, Y., Lei, J., ... & Shi, D. (2022). Systematic pan-cancer analysis of KLRB1 with prognostic value and immunological activity across human tumors. Journal of immunology research, 2022.

Di, W., Fan, W., Wu, F., Shi, Z., Wang, Z., Yu, M., ... & Zhang, W. (2022). Clinical characterization and immunosuppressive regulation of CD161 (KLRB1) in glioma through 916 samples. Cancer Science, 113(2), 756-769.

Hachim, M., Hachim, I., & Hannawi, S. (2022). Killer cell lectin-like receptor subfamily B member 1 (KLRB1), lymphocyte function-associated antigen 3 (CD58), and Niban1 (FAM129A) are unique markers for developed (central and effector) memory T cells in rheumatoid arthritis. Saudi Medical Journal, 43(6).

Lu, J., Bi, Y., Zhu, Y., Huipeng, S., Duan, W., & Zhou, J. (2021). CD3D, GZMK, and KLRB1 are potential markers for early diagnosis of rheumatoid arthritis, especially in anti-citrullinated protein antibody-negative patients. Frontiers in Pharmacology, 12, 726529.

Zhang, G., Liu, Y., Dong, F., & Liu, X. (2020). Transcription/expression of KLRB1 gene as a prognostic indicator in human esophageal squamous cell carcinoma. Combinatorial Chemistry & High Throughput Screening, 23(7), 667-674.

Thude, H., Rother, S., Sterneck, M., Klempnauer, J., Nashan, B., Schwinzer, R., & Koch, M. (2018). The killer cell lectin‐like receptor B1 (KLRB1) 503T> C polymorphism (rs1135816) and acute rejection after liver transplantation. Hla, 91(1), 52-55.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant KLRB1 protein, hFc Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-KLRB1 Recombinant Antibody (B199.2) (CAT#: CBMAB-K0167-LY)

Mouse Anti-KLRB1 Recombinant Antibody (28764) (CAT#: CBMAB-C0789-LY)

Mouse Anti-KLRB1 Recombinant Antibody (10/78) (CAT#: CBMAB-K0163-LY)

Mouse Anti-KLRB1 Recombinant Antibody (CBXC-2460) (CAT#: CBMAB-C1657-CQ)

Mouse Anti-KLRB1 Recombinant Antibody (14F2F11) (CAT#: CBMAB-K1397-LY)

Mouse Anti-KLRB1 Recombinant Antibody (3F8) (CAT#: CBMAB-C6074-LY)

Mouse Anti-KLRB1 Recombinant Antibody (B-D51) (CAT#: CBMAB-C5264WJ)

Mouse Anti-KLRB1 Recombinant Antibody (CBXC-2418) (CAT#: CBMAB-C0030-CQ)

Mouse Anti-KLRB1 Recombinant Antibody (CBFYC-0156) (CAT#: CBMAB-C0178-FY)

Mouse Anti-KLRB1 Recombinant Antibody (HP-3G10) (CAT#: CBMAB-K0164-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry