Human Recombinant KMT5A protein (V2LY-0526-LY6357)

Name: Human Recombinant KMT5A protein
SKU: V2LY-0526-LY6357
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Protein Construction

This product is Human Recombinant KMT5A protein consist of Amino Acid: 195-352 and predicts a molecular mass of 18.2 kDa.

Molecule Mass

18.2 kDa

Sequence

Amino Acid: 195-352

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Lysine Methyltransferase 5A

Function

Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.

Biological Process

Cell divisionIEA:UniProtKB-KW
Chromatin organizationIEA:UniProtKB-KW
Mitotic chromosome condensationTAS:Reactome
Negative regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIMP:UniProtKB
Negative regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:UniProtKB
Peptidyl-lysine monomethylationManual Assertion Based On ExperimentIDA:UniProtKB
Regulation of DNA damage response, signal transduction by p53 class mediatorManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of signal transduction by p53 class mediatorTAS:Reactome

Cellular Location

Nucleus
Chromosome
Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress-dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin.

PTM

Acetylated at Lys-172; does not change methyltransferase activity. Deacetylated at Lys-172 by SIRT2; does not change methyltransferase activity.
Ubiquitinated and degraded by the DCX(DTL) complex.

Crain, A. T., Klusza, S., Armstrong, R. L., Santa Rosa, P., Temple, B. R., Strahl, B. D., ... & Duronio, R. J. (2022). Distinct developmental phenotypes result from mutation of Set8/KMT5A and histone H4 lysine 20 in Drosophila melanogaster. Genetics, 221(2), iyac054.

Yu, B., Su, J., Shi, Q., Liu, Q., Ma, J., Ru, G., ... & Tang, J. (2022). KMT5A-methylated SNIP1 promotes triple-negative breast cancer metastasis by activating YAP signaling. Nature Communications, 13(1), 2192.

An, J., Zhang, J., Wang, Z., Wang, K., Liang, W., Liu, X., ... & Wang, S. (2022). KMT5A Knockdown Suppresses Osteosarcoma Cell Proliferation and Metastasis Through Ꞵ-Catenin Signalling. Clinical and Investigative Medicine, 45(3), E23-31.

Lu, X., Xu, M., Zhu, Q., Zhang, J., Liu, G., Bao, Y., ... & Zhu, W. G. (2021). RNF8‐ubiquitinated KMT5A is required for RNF168‐induced H2A ubiquitination in response to DNA damage. The FASEB Journal, 35(4), e21326.

Li, J., Qian, Y., Zhang, C., Wang, W., Qiao, Y., Song, H., ... & Deng, X. (2021). LncRNA LINC00473 is involved in the progression of invasive pituitary adenoma by upregulating KMT5A via ceRNA-mediated miR-502-3p evasion. Cell Death & Disease, 12(6), 580.

Lu, L., Li, X., Zhong, Z., Zhou, W., Zhou, D., Zhu, M., & Miao, C. (2021). KMT5A downregulation participated in High Glucose-mediated EndMT via Upregulation of ENO1 Expression in Diabetic Nephropathy. International Journal of Biological Sciences, 17(15), 4093.

Huang, T., Li, X., Wang, F., Lu, L., Hou, W., Zhu, M., & Miao, C. (2021). The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy. Cell Death & Disease, 12(4), 333.

Lu, L., Zhong, Z., Gu, J., Nan, K., Zhu, M., & Miao, C. (2021). ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy. Molecular Medicine, 27(1), 1-19.

Lin, Z. Z., Ming, D. S., Chen, Y. B., Zhang, J. M., Chen, H. H., Jiang, J. J., & Zhang, Z. S. (2019). KMT5A promotes metastasis of clear cell renal cell carcinoma through reducing cadherin‑1 expression. Oncology Letters, 17(6), 4907-4913.

Liao, T., Wang, Y. J., Hu, J. Q., Wang, Y., Han, L. T., Ma, B., ... & Ji, Q. H. (2018). Histone methyltransferase KMT5A gene modulates oncogenesis and lipid metabolism of papillary thyroid cancer in vitro. Oncology reports, 39(5), 2185-2192.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant KMT5A protein, GST Tag (CAT#: V2LY-0526-LY6358)

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For research use only. Not intended for any clinical use.

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