Human Recombinant KREMEN1 protein, His Tag (V2LY-0526-LY5217)

Name: Human Recombinant KREMEN1 protein, His Tag
SKU: V2LY-0526-LY5217
Rating: 5 (1 reviews)

See all KREMEN1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant KREMEN1 protein, His Tag consist of Amino Acid: 1-394 and predicts a molecular mass of 42.6 kDa.

Molecule Mass

42.6 kDa

Sequence

Amino Acid: 1-394

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

kringle containing transmembrane protein 1

Function

Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6. In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane. Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1. Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation. Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification.

Biological Process

Apoptotic processISS:UniProtKB
Cell communicationManual Assertion Based On ExperimentTAS:UniProtKB
Limb developmentISS:UniProtKB
Negative regulation of axon regenerationIEA:Ensembl
Negative regulation of canonical Wnt signaling pathwayISS:UniProtKB
Negative regulation of ossificationIEA:Ensembl
Regulation of canonical Wnt signaling pathway1 PublicationNAS:BHF-UCL
Wnt signaling pathwayIEA:UniProtKB-KW

Cellular Location

Cell membrane

Involvement in disease

Ectodermal dysplasia 13, hair/tooth type (ECTD13):
A form of ectodermal dysplasia, a disorder due to abnormal development of two or more ectodermal structures. ECTD13 is an autosomal recessive form characterized by severe oligodontia accompanied by anomalies of hair and skin.

Topology

Extracellular: 21-392
Helical: 393-413
Cytoplasmic: 414-473

Li, X., Liu, Z., Yan, X., Tian, Y., Liu, K., Zhao, Y., ... & Zhang, C. (2023). VP2 residue N142 of coxsackievirus A10 is critical for the interaction with KREMEN1 receptor and neutralizing antibodies and the pathogenicity in mice. Plos Pathogens, 19(10), e1011662.

Gu, Y., Cao, J., Zhang, X., Gao, H., Wang, Y., Wang, J., ... & Lu, Z. (2022). Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2. Cell research, 32(1), 24-37.

Hoffmann, M., & Pöhlmann, S. (2022). Novel sars-Cov-2 receptors: Asgr1 and Kremen1. Cell Research, 32(1), 1-2.

Xu, Y., Nowrangi, D., Liang, H., Wang, T., Yu, L., Lu, T., ... & Tang, J. (2020). DKK3 attenuates JNK and AP-1 induced inflammation via Kremen-1 and DVL-1 in mice following intracerebral hemorrhage. Journal of Neuroinflammation, 17(1), 1-14.

Zhao, Y., Zhou, D., Ni, T., Karia, D., Kotecha, A., Wang, X., ... & Stuart, D. I. (2020). Hand-foot-and-mouth disease virus receptor KREMEN1 binds the canyon of Coxsackie Virus A10. Nature communications, 11(1), 38.

Sumia, I., Pierani, A., & Causeret, F. (2019). Kremen1-induced cell death is regulated by homo-and heterodimerization. Cell death discovery, 5(1), 91.

Wang, H., Lu, B., & Chen, J. (2019). Knockdown of lncRNA SNHG1 attenuated Aβ25-35-inudced neuronal injury via regulating KREMEN1 by acting as a ceRNA of miR-137 in neuronal cells. Biochemical and biophysical research communications, 518(3), 438-444.

Staring, J., van den Hengel, L. G., Raaben, M., Blomen, V. A., Carette, J. E., & Brummelkamp, T. R. (2018). KREMEN1 is a host entry receptor for a major group of enteroviruses. Cell Host & Microbe, 23(5), 636-643.

Ross, S. P., Baker, K. E., Fisher, A., Hoff, L., Pak, E. S., & Murashov, A. K. (2018). miRNA-431 prevents amyloid-β-induced synapse loss in neuronal cell culture model of Alzheimer's disease by silencing kremen1. Frontiers in cellular neuroscience, 12, 87.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant KREMEN1 protein, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-KREMEN1 Recombinant Antibody (301324) (CAT#: CBMAB-K1529-LY)

Rat Anti-KREMEN1 Recombinant Antibody (14L675) (CAT#: CBMAB-K1526-LY)

Mouse Anti-KREMEN1 Recombinant Antibody (1E7) (CAT#: CBMAB-A4808-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry