Human Recombinant LGR4 protein, ECD, hFc Tag (V2LY-0526-LY5289)

Name: Human Recombinant LGR4 protein, ECD, hFc Tag
SKU: V2LY-0526-LY5289
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant LGR4 protein, ECD, hFc Tag consist of Amino Acid: 1-544 and predicts a molecular mass of 83.92 kDa.

Molecule Mass

83.92 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-544

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glycerol, DTT

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

leucine-rich repeat-containing G protein-coupled receptor 4

Function

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP (By similarity).

Biological Process

Activation of adenylate cyclase activityManual Assertion Based On ExperimentIBA:GO_Central
Adenylate cyclase-activating G protein-coupled receptor signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Bone mineralizationISS:UniProtKB
Bone remodelingISS:UniProtKB
Canonical Wnt signaling pathway involved in metanephric kidney developmentIEA:Ensembl
Cell differentiation involved in metanephros developmentIEA:Ensembl
Circadian regulation of gene expressionISS:UniProtKB
Digestive tract developmentIEA:Ensembl
Epithelial cell proliferation involved in renal tubule morphogenesisIEA:Ensembl
Hair follicle developmentIEA:Ensembl
Hormone-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Innate immune responseIEA:UniProtKB-KW
Intestinal stem cell homeostasisIEA:Ensembl
Male genitalia developmentIEA:Ensembl
Metanephric glomerulus developmentIEA:Ensembl
Metanephric nephron tubule morphogenesisIEA:Ensembl
Negative regulation of cold-induced thermogenesisBy SimilarityISS:YuBioLab
Negative regulation of cytokine productionISS:UniProtKB
Negative regulation of toll-like receptor signaling pathwayISS:UniProtKB
Negative regulation of transcription, DNA-templatedIEA:Ensembl
Osteoblast differentiationISS:UniProtKB
Positive regulation of branching involved in ureteric bud morphogenesisIEA:Ensembl
Positive regulation of canonical Wnt signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedIEA:Ensembl
SpermatogenesisISS:UniProtKB

Cellular Location

Cell membrane

Involvement in disease

Osteoporosis (OSTEOP):
A systemic skeletal disorder characterized by decreased bone mass and deterioration of bone microarchitecture without alteration in the composition of bone. The result is fragile bones and an increased risk of fractures, even after minimal trauma. Osteoporosis is a chronic condition of multifactorial etiology and is usually clinically silent until a fracture occurs.

Topology

Extracellular: 25-544
Helical: 545-565
Cytoplasmic: 566-575
Helical: 576-596
Extracellular: 597-620
Helical: 621-641
Cytoplasmic: 642-661
Helical: 662-682
Extracellular: 683-703
Helical: 704-724
Cytoplasmic: 725-756
Helical: 757-777
Extracellular: 778-783
Helical: 784-804
Cytoplasmic: 805-951

Saponara, E., Penno, C., Orsini, V., Wang, Z. Y., Fischer, A., Aebi, A., ... & Ruffner, H. (2023). Loss of Hepatic Leucine-Rich Repeat-Containing G-Protein Coupled Receptors 4 and 5 Promotes Nonalcoholic Fatty Liver Disease. The American Journal of Pathology, 193(2), 161-181.

Shimamura, M., Hayashi, H., Ju, N., Yoshida, S., Baba, S., Sasaki, T., ... & Nakagami, H. (2023). R-spondin 3/LGR4 (Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 4) Axis Is a Novel Inflammatory and Neurite Outgrowth Signaling System in the Ischemic Brain in Mice. Stroke, 54(6), 1606-1615.

Bi, Y., Zhang, L., Song, Y., Sun, L., Mulholland, M. W., Yin, Y., & Zhang, W. (2023). Rspo2-LGR4 exacerbates hepatocellular carcinoma progression via activation of Wnt/β-catenin signaling pathway. Gastroenterología y Hepatología.

Filipowska, J., Kondegowda, N. G., Leon-Rivera, N., Dhawan, S., & Vasavada, R. C. (2022). LGR4, a G protein-coupled receptor with a systemic role: from development to metabolic regulation. Frontiers in Endocrinology, 13, 867001.

Carrillo-López, N., Martínez-Arias, L., Alonso-Montes, C., Martín-Carro, B., Martín-Vírgala, J., Ruiz-Ortega, M., ... & Panizo, S. (2021). The receptor activator of nuclear factor κΒ ligand receptor leucine-rich repeat-containing G-protein-coupled receptor 4 contributes to parathyroid hormone-induced vascular calcification. Nephrology Dialysis Transplantation, 36(4), 618-631.

Lee, Y. H., Sharma, A. R., Jagga, S., Lee, S. S., & Nam, J. S. (2021). Differential expression patterns of rspondin family and leucine-rich repeat-containing G-protein coupled receptors in chondrocytes and osteoblasts. Cell Journal (Yakhteh), 22(4), 437.

Cui, J., Toh, Y., Park, S., Yu, W., Tu, J., Wu, L., ... & Liu, Q. J. (2021). Drug conjugates of antagonistic R-spondin 4 mutant for simultaneous targeting of Leucine-rich repeat-containing G protein-coupled receptors 4/5/6 for cancer treatment. Journal of Medicinal Chemistry, 64(17), 12572-12581.

Liu, X., & Xu, X. (2018). MicroRNA-137 dysregulation predisposes to osteoporotic fracture by impeding ALP activity and expression via suppression of leucine-rich repeat-containing G-protein-coupled receptor 4 expression. International journal of molecular medicine, 42(2), 1026-1033.

Da Silva, F., Massa, F., Motamedi, F. J., Vidal, V., Rocha, A. S., Gregoire, E. P., ... & Schedl, A. (2018). Myocardial-specific R-spondin3 drives proliferation of the coronary stems primarily through the Leucine Rich Repeat G Protein coupled receptor LGR4. Developmental biology, 441(1), 42-51.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant LGR4-VLP protein, His & AVI Tag (CAT#: V2LY-0526-LY5290)

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For research use only. Not intended for any clinical use.

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