Human Recombinant LILRB2 protein, ECD, His Tag (V2LY-0526-LY4986)

Name: Human Recombinant LILRB2 protein, ECD, His Tag
SKU: V2LY-0526-LY4986
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant LILRB2 protein, ECD, His Tag consist of Amino Acid: 1-461 and predicts a molecular mass of 49.2 kDa.

Molecule Mass

49.2 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-461

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 2

Function

Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042).
Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:20448110, PubMed:27859042, PubMed:16455647).
Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885).

Biological Process

Adaptive immune responseIEA:UniProtKB-KW
Cell surface receptor signaling pathwayManual Assertion Based On ExperimentTAS:ProtInc
Cell-cell signalingManual Assertion Based On ExperimentTAS:ProtInc
Cellular defense responseManual Assertion Based On ExperimentTAS:ProtInc
Cellular response to lipopolysaccharideManual Assertion Based On ExperimentIMP:BHF-UCL
Cytokine-mediated signaling pathwayManual Assertion Based On ExperimentIBA:GO_Central
Fc receptor mediated inhibitory signaling pathwayManual Assertion Based On ExperimentIDA:UniProtKB
Heterotypic cell-cell adhesionManual Assertion Based On ExperimentIDA:BHF-UCL
Immune responseManual Assertion Based On ExperimentTAS:ProtInc
Immune response-inhibiting cell surface receptor signaling pathwayManual Assertion Based On ExperimentIDA:BHF-UCL
Interleukin-10-mediated signaling pathwayManual Assertion Based On ExperimentIEP:ARUK-UCL
Learning or memoryBy SimilarityISS:ARUK-UCL
Negative regulation of antigen processing and presentationManual Assertion Based On ExperimentIDA:BHF-UCL
Negative regulation of calcium ion transportManual Assertion Based On ExperimentIDA:UniProtKB
Negative regulation of postsynaptic density organizationIC:ARUK-UCL
Negative regulation of protein metabolic processBy SimilarityISS:ARUK-UCL
Negative regulation of T cell costimulationManual Assertion Based On ExperimentIMP:ARUK-UCL
Negative regulation of T cell proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of interleukin-6 production1 PublicationNAS:BHF-UCL
Positive regulation of long-term synaptic depressionBy SimilarityISS:ARUK-UCL
Positive regulation of protein dephosphorylationBy SimilarityISS:ARUK-UCL
Positive regulation of regulatory T cell differentiationManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of T cell proliferationManual Assertion Based On ExperimentIDA:BHF-UCL
Positive regulation of T cell tolerance inductionManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of tolerance inductionManual Assertion Based On ExperimentIMP:UniProtKB
Regulation of dendritic cell differentiation1 PublicationIC:BHF-UCL
Regulation of long-term synaptic potentiationBy SimilarityISS:ARUK-UCL
Signal transductionManual Assertion Based On ExperimentIDA:BHF-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 22-460
Helical: 461-481
Cytoplasmic: 482-597

PTM

Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6.

Umiker, B., Hashambhoy-Ramsay, Y., Smith, J., Rahman, T., Mueller, A., Davidson, R., ... & Shaffer, D. R. (2023). Inhibition of LILRB2 by a Novel Blocking Antibody Designed to Reprogram Immunosuppressive Macrophages to Drive T-Cell Activation in Tumors. Molecular Cancer Therapeutics, 22(4), 471-484.

Zhao, P., Xu, Y., Jiang, L. L., Fan, X., Ku, Z., Li, L., ... & An, Z. (2022). LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions. Molecular neurodegeneration, 17(1), 44.

Nishiyama, S., Hirose, N., Yanoshita, M., Takano, M., Kubo, N., Yamauchi, Y., ... & Tanimoto, K. (2021). ANGPTL2 induces synovial inflammation via LILRB2. Inflammation, 44, 1108-1118.

Wang, Q., Song, H., Cheng, H., Qi, J., Nam, G., Tan, S., ... & Gao, G. F. (2020). Structures of the four Ig-like domain LILRB2 and the four-domain LILRB1 and HLA-G1 complex. Cellular & Molecular Immunology, 17(9), 966-975.

Yanovsky, R. L., Chen, H., Leslie, S., Carrington, M., & Liao, W. (2020). The interaction of LILRB2 with HLA-B is associated with psoriasis susceptibility. The Journal of investigative dermatology, 140(6), 1292.

Shao, H., Ma, L., Jin, F., Zhou, Y., Tao, M., & Teng, Y. (2018). Immune inhibitory receptor LILRB2 is critical for the endometrial cancer progression. Biochemical and biophysical research communications, 506(1), 243-250.

Chen, H. M., van der Touw, W., Wang, Y. S., Kang, K., Mai, S., Zhang, J., ... & Chen, S. H. (2018). Blocking immunoinhibitory receptor LILRB2 reprograms tumor-associated myeloid cells and promotes antitumor immunity. The Journal of clinical investigation, 128(12), 5647-5662.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant LILRB2 protein, ECD, hFc Tag (CAT#: V2LY-0526-LY4984)

Human Recombinant LILRB2 protein, ECD, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY4985)

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Isotype control

For research use only. Not intended for any clinical use.

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