Human Recombinant MAP3K8 protein, GST Tag-1 (V2LY-0526-LY5444)

Name: Human Recombinant MAP3K8 protein, GST Tag-1
SKU: V2LY-0526-LY5444
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MAP3K8 protein, GST Tag consist of Amino Acid: 30-397 and predicts a molecular mass of 68 kDa.

Molecule Mass

68 kDa

Sequence

Amino Acid: 30-397

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>91% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 8

Function

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

Biological Process

Cell cycleIEA:UniProtKB-KW
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
T cell costimulationTAS:Reactome

Cellular Location

Cytoplasm

PTM

Autophosphorylated (PubMed:8226782, PubMed:1833717).
Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues (PubMed:8226782).
Phosphorylated on Thr-290; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation (PubMed:15466476, PubMed:15699325).
Phosphorylated on Ser-400 by IKBKB; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages (PubMed:17472361, PubMed:22988300).

Zhang, H., Deng, J., Huang, K., He, Y., Cai, Z., & He, Y. (2022). circNup188/miR-760–3p/Map3k8 axis regulates inflammation in cerebral ischemia. Molecular and cellular probes, 64, 101830.

Ren, J., Xu, Y., Liu, J., Wu, S., Zhang, R., Cao, H., & Sun, J. (2021). MAP3K8 is a prognostic biomarker and correlated with immune response in glioma. Frontiers in Molecular Biosciences, 8, 779290.

Hao, J., Cao, Y., Yu, H., Zong, L., An, R., & Xue, Y. (2021). Effect of MAP3K8 on prognosis and tumor-related inflammation in renal clear cell carcinoma. Frontiers in genetics, 12, 674613.

Zannikou, M., Barbayianni, I., Fanidis, D., Grigorakaki, T., Vlachopoulou, E., Konstantopoulos, D., ... & Aidinis, V. (2021). MAP3K8 Regulates Cox-2–Mediated Prostaglandin E2 Production in the Lung and Suppresses Pulmonary Inflammation and Fibrosis. The Journal of Immunology, 206(3), 607-620.

Li, J., Lv, H., & Che, Y. (2020). microRNA-381-3p confers protection against ischemic stroke through promoting angiogenesis and inhibiting inflammation by suppressing Cebpb and Map3k8. Cellular and molecular neurobiology, 40, 1307-1319.

Houlier, A., Pissaloux, D., Masse, I., Tirode, F., Karanian, M., Pincus, L. B., ... & de La Fouchardiere, A. (2020). Melanocytic tumors with MAP3K8 fusions: report of 33 cases with morphological-genetic correlations. Modern Pathology, 33(5), 846-857.

Zhang, D., Liu, Y., Cui, Y., & Cui, S. (2019). Mitogen-activated protein kinase kinase kinase 8 (MAP3K8) mediates the LH-induced stimulation of progesterone synthesis in the porcine corpus luteum. Reproduction, Fertility and Development, 31(9), 1444-1456.

Gianì, F., Russo, G., Pennisi, M., Sciacca, L., Frasca, F., & Pappalardo, F. (2019). Computational modeling reveals MAP3K8 as mediator of resistance to vemurafenib in thyroid cancer stem cells. Bioinformatics, 35(13), 2267-2275.

Newman, S., Fan, L., Pribnow, A., Silkov, A., Rice, S. V., Lee, S., ... & Bahrami, A. (2019). Clinical genome sequencing uncovers potentially targetable truncations and fusions of MAP3K8 in spitzoid and other melanomas. Nature medicine, 25(4), 597-602.

Lehmann, B. D., Shaver, T. M., Johnson, D. B., Li, Z., Gonzalez-Ericsson, P. I., Sánchez, V., ... & Pietenpol, J. A. (2019). Identification of targetable recurrent MAP3K8 rearrangements in melanomas lacking known driver mutations. Molecular Cancer Research, 17(9), 1842-1853.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MAP3K8 protein, GST Tag (CAT#: V2LY-0526-LY5443)

Human Recombinant MAP3K8, Active protein, GST Tag (CAT#: V2LY-0526-LY5445)

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Human Recombinant MAP3K8 protein, GST Tag (CAT#: V2LY-0526-LY5443)

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Human Recombinant MAP3K8, Active protein, GST Tag (CAT#: V2LY-0526-LY5445)

Isotype control

For research use only. Not intended for any clinical use.

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