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Rabbit Anti-MAP3K8 Recombinant Antibody (3H18L5) (CBMAB-C6267-LY)

This product is antibody recognizes MAP3K8. The antibody 3H18L5 immunoassay techniques such as: WB.
See all MAP3K8 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
3H18L5
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Peptide corresponding to amino acids 282-293 of human COT/TPL2/MAP3K8
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Preservative
0.09% sodium azide
Concentration
0.5 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 8
Introduction
This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]
Entrez Gene ID
1326
UniProt ID
P41279
Alternative Names
Mitogen-Activated Protein Kinase Kinase Kinase 8; Tumor Progression Locus 2; Proto-Oncogene C-Cot; Tpl-2; ESTF; COT; Proto-Oncogene Serine/Threoine Protein Kinase; Augmented In Rheumatoid Arthritis 2; Cot (Cancer Osaka Thyroid) Oncogene; Serine/Threonine-Protein Kinase Cot;
Function
Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.
Biological Process
Cell cycleIEA:UniProtKB-KW
Protein phosphorylationManual Assertion Based On ExperimentTAS:ProtInc
T cell costimulationTAS:Reactome
Cellular Location
Cytoplasm
PTM
Autophosphorylated (PubMed:8226782, PubMed:1833717).
Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues (PubMed:8226782).
Phosphorylated on Thr-290; the phosphorylation is necessary but not sufficient for full kinase activity in vitro and for the dissociation of isoform 1 from NFKB1, leading to its degradation (PubMed:15466476, PubMed:15699325).
Phosphorylated on Ser-400 by IKBKB; the phosphorylation is required for LPS-stimulated activation of the MAPK/ERK pathway in macrophages (PubMed:17472361, PubMed:22988300).

Zhang, H., Deng, J., Huang, K., He, Y., Cai, Z., & He, Y. (2022). circNup188/miR-760–3p/Map3k8 axis regulates inflammation in cerebral ischemia. Molecular and cellular probes, 64, 101830.

Ren, J., Xu, Y., Liu, J., Wu, S., Zhang, R., Cao, H., & Sun, J. (2021). MAP3K8 is a prognostic biomarker and correlated with immune response in glioma. Frontiers in Molecular Biosciences, 8, 779290.

Hao, J., Cao, Y., Yu, H., Zong, L., An, R., & Xue, Y. (2021). Effect of MAP3K8 on prognosis and tumor-related inflammation in renal clear cell carcinoma. Frontiers in genetics, 12, 674613.

Zannikou, M., Barbayianni, I., Fanidis, D., Grigorakaki, T., Vlachopoulou, E., Konstantopoulos, D., ... & Aidinis, V. (2021). MAP3K8 Regulates Cox-2–Mediated Prostaglandin E2 Production in the Lung and Suppresses Pulmonary Inflammation and Fibrosis. The Journal of Immunology, 206(3), 607-620.

Li, J., Lv, H., & Che, Y. (2020). microRNA-381-3p confers protection against ischemic stroke through promoting angiogenesis and inhibiting inflammation by suppressing Cebpb and Map3k8. Cellular and molecular neurobiology, 40, 1307-1319.

Houlier, A., Pissaloux, D., Masse, I., Tirode, F., Karanian, M., Pincus, L. B., ... & de La Fouchardiere, A. (2020). Melanocytic tumors with MAP3K8 fusions: report of 33 cases with morphological-genetic correlations. Modern Pathology, 33(5), 846-857.

Zhang, D., Liu, Y., Cui, Y., & Cui, S. (2019). Mitogen-activated protein kinase kinase kinase 8 (MAP3K8) mediates the LH-induced stimulation of progesterone synthesis in the porcine corpus luteum. Reproduction, Fertility and Development, 31(9), 1444-1456.

Gianì, F., Russo, G., Pennisi, M., Sciacca, L., Frasca, F., & Pappalardo, F. (2019). Computational modeling reveals MAP3K8 as mediator of resistance to vemurafenib in thyroid cancer stem cells. Bioinformatics, 35(13), 2267-2275.

Newman, S., Fan, L., Pribnow, A., Silkov, A., Rice, S. V., Lee, S., ... & Bahrami, A. (2019). Clinical genome sequencing uncovers potentially targetable truncations and fusions of MAP3K8 in spitzoid and other melanomas. Nature medicine, 25(4), 597-602.

Lehmann, B. D., Shaver, T. M., Johnson, D. B., Li, Z., Gonzalez-Ericsson, P. I., Sánchez, V., ... & Pietenpol, J. A. (2019). Identification of targetable recurrent MAP3K8 rearrangements in melanomas lacking known driver mutations. Molecular Cancer Research, 17(9), 1842-1853.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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