Human Recombinant MEK2 protein, GST Tag-1 (V2LY-0526-LY5524)

Name: Human Recombinant MEK2 protein, GST Tag-1
SKU: V2LY-0526-LY5524
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MEK2 protein, GST Tag consist of Amino Acid: 1-400 and predicts a molecular mass of 70.7 kDa.

Molecule Mass

70.7 kDa

Sequence

Amino Acid: 1-400

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>92% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Mitogen-Activated Protein Kinase Kinase 2

Alternative Names

Mitogen-Activated Protein Kinase Kinase 2; ERK Activator Kinase 2; MAP Kinase Kinase 2; MAPK/ERK Kinase 2; EC 2.7.12.2; PRKMK2; MEK2; MKK2;

Function

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126).

Biological Process

ERK1 and ERK2 cascadeManual Assertion Based On ExperimentIMP:BHF-UCL
MAPK cascadeManual Assertion Based On ExperimentIBA:GO_Central
Peptidyl-serine autophosphorylationManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of production of miRNAs involved in gene silencing by miRNAManual Assertion Based On ExperimentIMP:BHF-UCL
Positive regulation of protein serine/threonine kinase activityManual Assertion Based On ExperimentIDA:UniProtKB
Positive regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIMP:BHF-UCL
Regulation of early endosome to late endosome transportManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of Golgi inheritanceManual Assertion Based On ExperimentTAS:UniProtKB
Regulation of stress-activated MAPK cascadeManual Assertion Based On ExperimentTAS:UniProtKB

Cellular Location

Cytoplasm
Membrane
Membrane localization is probably regulated by its interaction with KSR1.

Involvement in disease

Cardiofaciocutaneous syndrome 4 (CFC4):
A form of cardiofaciocutaneous syndrome, a multiple congenital anomaly disorder characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices.

PTM

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).
(Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.

Sarkar, A., Chamucero-Millares, J. A., & Rojas, M. (2022). Romulus and Remus of Inflammation: The Conflicting Roles of MAP2K1 and MAP2K2 in Acute Respiratory Distress Syndrome. American Journal of Respiratory Cell and Molecular Biology, 66(5), 479-480.

Hua, Z., Wei, R., Guo, M., Lin, Z., Yu, X., Li, X., ... & Yang, Y. (2022). YTHDF2 promotes multiple myeloma cell proliferation via STAT5A/MAP2K2/p-ERK axis. Oncogene, 41(10), 1482-1491.

Gong, K. Q., Mikacenic, C., Long, M. E., Frevert, C. W., Birkland, T. P., Charron, J., ... & Manicone, A. M. (2022). MAP2K2 delays recovery in murine models of acute lung injury and associates with acute respiratory distress syndrome outcome. American Journal of Respiratory Cell and Molecular Biology, 66(5), 555-563.

Ren, L. X., Qi, J. C., Zhao, A. N., Shi, B., Zhang, H., Wang, D. D., & Yang, Z. (2021). Myc-associated zinc-finger protein promotes clear cell renal cell carcinoma progression through transcriptional activation of the MAP2K2-dependent ERK pathway. Cancer cell international, 21(1), 1-13.

Xu, J., Xiong, H., Zhao, Z., Luo, M., Ju, Y., Yang, G., & Mei, Z. (2021). Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway. Food & Function, 12(10), 4556-4569.

Jacinto, J. G., Häfliger, I. M., Gentile, A., & Drögemüller, C. (2021). A heterozygous missense variant in MAP2K2 in a stillborn romagnola calf with skeletal-cardio-enteric dysplasia. Animals, 11(7), 1931.

Richmond, C. S., Vallatharasu, Y., Deviley, J. A., Vos, C. R., Parsons, B. M., & Kenny, P. A. (2019). Sequential treatment failures in response to BRAF/MEK and immune checkpoint inhibitors mediated by MAP2K2 and B2M mutations in melanoma. Experimental and Molecular Pathology, 110, 104260.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MEK2 protein, GST Tag (CAT#: V2LY-0526-LY5521)

Human Recombinant MEK2, Active protein, GST Tag (CAT#: V2LY-0526-LY5522)

Human Recombinant MEK2 (S222E, S226D) protein, His Tag (CAT#: V2LY-0526-LY5523)

MAP2K2 Matched Antibody Pair (712) (CAT#: APMAB-712LY)

Antibody Pairs

MAP2K2 Matched Antibody Pair (712) (CAT#: APMAB-712LY)

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Isotype control

For research use only. Not intended for any clinical use.

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