Human Recombinant MGMT protein, His Tag (V2LY-0526-LY5576)

Name: Human Recombinant MGMT protein, His Tag
SKU: V2LY-0526-LY5576
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MGMT protein, His Tag consist of Amino Acid: Full Length and predicts a molecular mass of 25 kDa.

Molecule Mass

25 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Sodium phosphate, NaCl, Imidazole, DTT, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

O-6-Methylguanine-DNA Methyltransferase

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Biological Process

Cellular response to ionizing radiation Source: Ensembl
Cellular response to organic cyclic compound Source: Ensembl
Cellular response to oxidative stress Source: Ensembl
DNA dealkylation involved in DNA repair Source: Ensembl
DNA ligation Source: ProtInc
DNA repair Source: GO_Central
Mammary gland epithelial cell differentiation Source: Ensembl
Negative regulation of apoptotic process Source: BHF-UCL
Positive regulation of double-strand break repair Source: BHF-UCL
Regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: Ensembl
Response to ethanol Source: Ensembl
Response to folic acid Source: Ensembl
Response to toxic substance Source: Ensembl
Response to xenobiotic stimulus Source: Ensembl

Cellular Location

Nucleus

Chen, Y., Qu, W., Tu, J., & Qi, H. (2023). Implications of Advances in Studies of O6-Methylguanine-DNA-Methyltransferase for Tumor Prognosis and Treatment. Frontiers in Bioscience-Landmark, 28(9), 197.

Chen, X., Sun, J., Li, Y., Jiang, W., Li, Z., Mao, J., ... & Tan, G. (2023). Proteomic and metabolomic analyses illustrate the mechanisms of expression of the O6‐methylguanine‐DNA methyltransferase gene in glioblastoma. CNS Neuroscience & Therapeutics.

Kostka, T., Empl, M. T., Seiwert, N., Geisen, S. M., Hoffmann, P., Adam, J., ... & Steinberg, P. (2021). Repair of O 6-carboxymethylguanine adducts by O 6-methylguanine-DNA methyltransferase in human colon epithelial cells. Carcinogenesis, 42(8), 1110-1118.

McAleenan, A., Kelly, C., Spiga, F., Kernohan, A., Cheng, H. Y., Dawson, S., ... & Kurian, K. M. (2021). Prognostic value of test (s) for O6‐methylguanine–DNA methyltransferase (MGMT) promoter methylation for predicting overall survival in people with glioblastoma treated with temozolomide. Cochrane Database of Systematic Reviews, (3).

Yu, W., Zhang, L., Wei, Q., & Shao, A. (2020). O6-methylguanine-DNA methyltransferase (MGMT): challenges and new opportunities in glioma chemotherapy. Frontiers in oncology, 9, 1547.

Dovek, L., Nguyen, N. T., Ozer, B. H., Li, N., Elashoff, R. M., Green, R. M., ... & Lai, A. (2019). Correlation of commercially available quantitative MGMT (O-6-methylguanine-DNA methyltransferase) promoter methylation scores and GBM patient survival. Neuro-Oncology Practice, 6(3), 194-202.

Feldheim, J., Kessler, A. F., Monoranu, C. M., Ernestus, R. I., Löhr, M., & Hagemann, C. (2019). Changes of O6-methylguanine DNA methyltransferase (MGMT) promoter methylation in glioblastoma relapse—a meta-analysis type literature review. Cancers, 11(12), 1837.

Yang, L., Li, W., Zhao, Y., Zhong, S., Wang, X., Jiang, S., ... & Zhao, G. (2019). Computational study of novel natural inhibitors targeting O6-methylguanine-DNA methyltransferase. World neurosurgery, 130, e294-e306.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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