Human Recombinant MMP1 protein, His Tag (V2LY-0526-LY5610)

Name: Human Recombinant MMP1 protein, His Tag
SKU: V2LY-0526-LY5610
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MMP1 protein, His Tag consist of Amino Acid: 1-469 and predicts a molecular mass of 55 kDa.

Molecule Mass

55 kDa

Verified

HPLC

Sequence

Amino Acid: 1-469

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, Brij35, Arginine

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Matrix Metallopeptidase 1

Function

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:2557822, PubMed:2153297, PubMed:1645757).

In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369).

Biological Process

Cellular protein metabolic process Source: Reactome
Cellular response to UV-A Source: UniProtKB
Collagen catabolic process Source: GO_Central
Extracellular matrix disassembly Source: Reactome
Extracellular matrix organization Source: GO_Central
Positive regulation of protein-containing complex assembly Source: ParkinsonsUK-UCL
Proteolysis Source: ParkinsonsUK-UCL

Cellular Location

Extracellular matrix

PTM

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Tyrosine phosphorylated in platelets by PKDCC/VLK.

Bartold, K., Iskierko, Z., Borowicz, P., Noworyta, K., Lin, C. Y., Kalecki, J., ... & Kutner, W. (2022). Molecularly imprinted polymer-based extended-gate field-effect transistor (EG-FET) chemosensor for selective determination of matrix metalloproteinase-1 (MMP-1) protein. Biosensors and Bioelectronics, 208, 114203.

Alwan, I. T., & Ghali, K. H. (2021). Association Risk of Metalomatrix Proteinase Enzymes Levels (MMP-1, MMP-9 And MMP-13) with Development of Rheumatoid Arthritis. Annals of the Romanian Society for Cell Biology, 11369-11378.

Cárcel-Márquez, J., Cullell, N., Muiño, E., Gallego-Fabrega, C., Lledós, M., Ibañez, L., ... & Fernandez-Cadenas, I. (2021). Causal effect of MMP-1 (matrix metalloproteinase-1), MMP-8, and MMP-12 levels on ischemic stroke: a Mendelian randomization study.

Wang, M., Zhou, Y., Huang, W., Zeng, Y., & Li, X. (2020). Association between matrix metalloproteinase-1 (MMP-1) protein level and the risk of rheumatoid arthritis and osteoarthritis: a meta-analysis. Brazilian Journal of Medical and Biological Research, 54, e10366.

Balkhi, S., Mashayekhi, F., Salehzadeh, A., & Saedi, H. S. (2020). Matrix metalloproteinase (MMP)-1 and MMP-3 gene variations affect MMP-1 and-3 serum concentration and associates with breast cancer. Molecular Biology Reports, 47(12), 9637-9644.

Mohammadian, H., Sharifi, R., Amirdehi, S. R., Taheri, E., & Bedoustani, A. B. (2020). Matrix metalloproteinase MMP1 and MMP9 genes expression in breast cancer tissue. Gene Reports, 21, 100906.

Zamolo, G., Grahovac, M., Žauhar, G., Vučinić, D., Kovač, L., Brajenić, N., & Grahovac, B. (2020). Matrix metalloproteinases MMP‐1, MMP‐2, and MMP‐13 are overexpressed in primary nodular melanoma. Journal of cutaneous pathology, 47(2), 139-145.

Chang, Y. T., Chu, L. J., Liu, Y. C., Chen, C. J., Wu, S. F., Chen, C. H., ... & Yu, J. S. (2020). Verification of saliva matrix metalloproteinase-1 as a strong diagnostic marker of oral cavity cancer. Cancers, 12(8), 2273.

Szóstek-Mioduchowska, A., Słowińska, M., Pacewicz, J., Skarzynski, D. J., & Okuda, K. (2020). Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis. Scientific Reports, 10(1), 1119.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

MMP1 Matched Antibody Pair (740) (CAT#: APMAB-740LY)

Antibody Pairs

MMP1 Matched Antibody Pair (740) (CAT#: APMAB-740LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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