Human Recombinant MOB1B protein, GST Tag (V2LY-0526-LY5624)

Name: Human Recombinant MOB1B protein, GST Tag
SKU: V2LY-0526-LY5624
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant MOB1B protein, GST Tag consist of Amino Acid: 1-216 and predicts a molecular mass of 52.3 kDa.

Molecule Mass

52.3 kDa

Sequence

Amino Acid: 1-216

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MOB KINASE ACTIVATOR 1B

Function

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L.

Biological Process

Hippo signaling Source: UniProtKB
Positive regulation of protein phosphorylation Source: GO_Central
Regulation of protein autophosphorylation Source: UniProtKB
Signal transduction Source: GO_Central

Cellular Location

Nucleus
Cytoplasm

PTM

Phosphorylated by STK3/MST2 and STK4/MST1 and this phosphorylation enhances its binding to LATS1.

Pang, H., Wang, J., Wei, Q., Liu, J., Chu, X., Yuan, C., ... & Zhang, J. (2023). miR‐548ag functions as an oncogene by suppressing MOB1B in the development of obesity‐related endometrial cancer. Cancer Science, 114(4), 1507.

Yang, H. B., Li, Y., Li, X. H., Yan, Q. M., Han, X. Z., Cao, J., ... & Li, J. L. (2023). The compensatory increase of GLIS3 inhibited neuronal apoptosis through regulating MOB1b: a possible strategy for the functional recovery after spinal cord injury. Experimental Animals, 23-0041.

Zhang, Y., Yao, Y., Wang, Z., Lu, D., Zhang, Y., Adetula, A. A., ... & Tang, Z. (2022). MiR-743a-5p regulates differentiation of myoblast by targeting Mob1b in skeletal muscle development and regeneration. Genes & Diseases, 9(4), 1038-1048.

Fan, S., Price, T., Huang, W., Plue, M., Warren, J., Sundaramoorthy, P., ... & Kang, Y. (2020). PINK1‐dependent mitophagy regulates the migration and homing of multiple myeloma cells via the MOB1B‐mediated hippo‐YAP/TAZ pathway. Advanced Science, 7(5), 1900860.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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