Human Recombinant MRAP protein, hFc Tag (V2LY-0526-LY5634)

Name: Human Recombinant MRAP protein, hFc Tag
SKU: V2LY-0526-LY5634
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant MRAP protein, hFc Tag consist of Amino Acid: 59-172 and predicts a molecular mass of 40.9 kDa.

Molecule Mass

40.9 kDa

Sequence

Amino Acid: 59-172

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MELANOCORTIN 2 RECEPTOR ACCESSORY PROTEIN

Function

Modulator of melanocortin receptors (MC1R, MC2R, MC3R, MC4R and MC5R). Acts by increasing ligand-sensitivity of melanocortin receptors and enhancing generation of cAMP by the receptors. Required both for MC2R trafficking to the cell surface of adrenal cells and for signaling in response to corticotropin (ACTH). May be involved in the intracellular trafficking pathways in adipocyte cells.

Biological Process

Negative regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
Negative regulation of protein localization to plasma membrane Source: BHF-UCL
Positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: BHF-UCL
Protein localization to plasma membrane Source: BHF-UCL
Regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: GO_Central

Cellular Location

Plasma membrane
Cell membrane
Endoplasmic reticulum
Endoplasmic reticulum membrane
Note: The formation of antiparallel homo- and heterodimers suggest that N- and C-terminus can both localize in the cytoplasmic and extracellular parts, depending on the context (PubMed:20371771). Upon insulin stimulation, it is redistributed into spotty structures throughout the cytoplasm.

Involvement in disease

Glucocorticoid deficiency 2 (GCCD2):
A form of glucocorticoid deficiency, a rare autosomal recessive disorder characterized by resistance to ACTH action on the adrenal cortex, adrenal insufficiency and an inability of the adrenal cortex to produce cortisol. It usually presents in the neonatal period or in early childhood with episodes of hypoglycemia and other symptoms related to cortisol deficiency, including failure to thrive, recurrent illnesses or infections, convulsions, and shock. In a small number of patients hypoglycemia can be sufficiently severe and persistent that it leads to serious long-term neurological damage or death. The diagnosis is readily confirmed with a low plasma cortisol measurement in the presence of an elevated ACTH level, and normal aldosterone and plasma renin measurements.

Topology

Helical: 38-58

Ji, R. L., Jiang, S. S., & Tao, Y. X. (2022). Modulation of Canine Melanocortin-3 and-4 Receptors by Melanocortin-2 Receptor Accessory Protein 1 and 2. Biomolecules, 12(11), 1608.

Wang, X., Xue, S., Lei, X., Song, W., Li, L., Li, X., ... & Guo, J. (2022). Pharmacological evaluation of melanocortin 2 receptor accessory protein 2 on axolotl neural melanocortin signaling. Frontiers in Endocrinology, 13, 820896.

Ji, R. L., & Tao, Y. X. (2022). Regulation of melanocortin-3 and-4 receptors by isoforms of melanocortin-2 receptor accessory protein 1 and 2. Biomolecules, 12(2), 244.

Fullone, M. R., Maftei, D., Vincenzi, M., Lattanzi, R., & Miele, R. (2022). Identification of regions involved in the physical interaction between melanocortin receptor accessory protein 2 and prokineticin receptor 2. Biomolecules, 12(3), 474.

Dores, R. M., & Chapa, E. (2021). Hypothesis and Theory: Evaluating the co-evolution of the melanocortin-2 receptor and the accessory protein MRAP1. Frontiers in Endocrinology, 12, 747843.

Berruien, N. N., & Smith, C. L. (2020). Emerging roles of melanocortin receptor accessory proteins (MRAP and MRAP2) in physiology and pathophysiology. Gene, 757, 144949.

Chen, V., Bruno, A. E., Britt, L. L., Hernandez, C. C., Gimenez, L. E., Peisley, A., ... & Millhauser, G. L. (2020). Membrane orientation and oligomerization of the melanocortin receptor accessory protein 2. Journal of Biological Chemistry, 295(48), 16370-16379.

Soletto, L., Hernández-Balfagó, S., Rocha, A., Scheerer, P., Kleinau, G., & Cerdá-Reverter, J. M. (2019). Melanocortin receptor accessory protein 2-induced adrenocorticotropic hormone response of human melanocortin 4 receptor. Journal of the Endocrine Society, 3(2), 314-323.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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