Human Recombinant MS4A1 protein, Biotin Conjugated, TrxA Tag (V2LY-0526-LY2727)

Name: Human Recombinant MS4A1 protein, Biotin Conjugated, TrxA Tag
SKU: V2LY-0526-LY2727
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

TrxA Tag

Protein Construction

This product is Human Recombinant MS4A1 protein, Biotin Conjugated, TrxA Tag consist of Amino Acid: 141-188 and predicts a molecular mass of 23.9 kDa.

Molecule Mass

23.9 kDa

Conjugates

Biotin

Sequence

Amino Acid: 141-188

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>80% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Membrane Spanning 4-Domains A1

Alternative Names

Membrane Spanning 4-Domains A1; Membrane-Spanning 4-Domains, Subfamily A, Member 1; Leukocyte Surface Antigen Leu-16; CD20 Antigen; CD20; Bp35; Membrane-Spanning 4-Domains Subfamily A Member 1; B-Lymphocyte Cell-Surface Antigen B1; B-Lymphocyte Surface Antigen B1;

Function

B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:3925015, PubMed:7684739, PubMed:12920111).

Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:7684739, PubMed:12920111, PubMed:18474602).

Biological Process

B cell activation Source: UniProtKB
B cell differentiation Source: UniProtKB
B cell proliferation Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
Calcium ion import into cytosol Source: UniProtKB
Cell surface receptor signaling pathway Source: GO_Central
Humoral immune response Source: UniProtKB
Protein tetramerization Source: UniProtKB
Response to bacterium Source: Ensembl
Store-operated calcium entry Source: UniProtKB

Cellular Location

Plasma membrane
Cell membrane
Note: Constitutively associated with membrane rafts.

Involvement in disease

Immunodeficiency, common variable, 5 (CVID5):
A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

Topology

Cytoplasmic: 1-56
Helical: 57-78
Extracellular: 79-84
Helical: 85-105
Cytoplasmic: 106-120
Helical: 121-141
Extracellular: 142-188
Helical: 189-209
Cytoplasmic: 210-297

PTM

Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.

Xuan, Z., Liu, L., Zhang, G., Zheng, X., Jiang, J., Wang, K., & Huang, P. (2023). Novel cell subtypes of SPP1+ S100P+, MS4A1-SPP1+ S100P+ were key subpopulations in intrahepatic cholangiocarcinoma. Biochimica et Biophysica Acta (BBA)-General Subjects, 1867(9), 130420.

Song, Y., Zhang, Z., Zhang, B., & Zhang, W. (2022). CD8+ T cell-associated genes MS4A1 and TNFRSF17 are prognostic markers and inhibit the progression of colon cancer. Frontiers in Oncology, 12, 941208.

Li, S., & Fang, Y. (2022). MS4A1 as a potential independent prognostic factor of breast cancer related to lipid metabolism and immune microenvironment based on TCGA database analysis. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 28, e934597-1.

Mudd Jr, T. W., Lu, C., Klement, J. D., & Liu, K. (2021). MS4A1 expression and function in T cells in the colorectal cancer tumor microenvironment. Cellular immunology, 360, 104260.

Jiang, D., Mo, Q., Sun, X., Wang, X., Dong, M., Zhang, G., ... & Zhao, Q. (2021). Pyruvate dehydrogenase kinase 4‐mediated metabolic reprogramming is involved in rituximab resistance in diffuse large B‐cell lymphoma by affecting the expression of MS4A1/CD20. Cancer Science, 112(9), 3585-3597.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

MS4A1 Matched Antibody Pair (753) (CAT#: APMAB-753LY)

MS4A1 Matched Antibody Pair (754) (CAT#: APMAB-754LY)

MS4A1 Matched Antibody Pair (755) (CAT#: APMAB-755LY)

Human Recombinant MS4A1-VLP protein (CAT#: V2LY-0526-LY155)

Canine Recombinant MS4A1-VLP protein, His Tag (CAT#: V2LY-0526-LY1217)

Cynomolgus Recombinant MS4A1 protein, His Tag (CAT#: V2LY-0526-LY1414)

Cynomolgus Recombinant MS4A1-VLP protein, His Tag (CAT#: V2LY-0526-LY1415)

Feline Recombinant MS4A1-VLP protein, His Tag (CAT#: V2LY-0526-LY1784)

Ferret Recombinant MS4A1 protein, His Tag (CAT#: V2LY-0526-LY1797)

Human Recombinant MS4A1 protein, His Tag (CAT#: V2LY-0526-LY2722)

Antibody Pairs

MS4A1 Matched Antibody Pair (753) (CAT#: APMAB-753LY)

MS4A1 Matched Antibody Pair (754) (CAT#: APMAB-754LY)

MS4A1 Matched Antibody Pair (755) (CAT#: APMAB-755LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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