Human Recombinant MSLN protein, Biotin Conjugated, His Tag (V2LY-0526-LY5551)

Name: Human Recombinant MSLN protein, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY5551
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MSLN protein, Biotin Conjugated, His Tag consist of Amino Acid: 1-286 and predicts a molecular mass of 28.2 kDa.

Molecule Mass

28.2 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-286

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Mesothelin

Function

Membrane-anchored forms may play a role in cellular adhesion.

Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Biological Process

Cell adhesion Source: UniProtKB
Cell-matrix adhesion Source: GO_Central
Pancreas development Source: Ensembl

Cellular Location

Plasma membrane
Cell membrane
Golgi apparatus
Megakaryocyte-potentiating factor:
Secreted
Isoform 3:
Secreted

Involvement in disease

Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.

PTM

Both MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.

Hagerty, B. L., & Takabe, K. (2023). Biology of Mesothelin and Clinical Implications: A Review of Existing Literature. World Journal of Oncology, 14(5), 340.

Wang, Q., & Gong, R. (2022). Immunotherapy targeting mesothelin in acute myeloid leukemia. Journal of Leukocyte Biology, 112(4), 813-821.

Schoutrop, E., El-Serafi, I., Poiret, T., Zhao, Y., Gultekin, O., He, R., ... & Mattsson, J. (2021). Mesothelin-specific CAR T cells target ovarian cancer. Cancer Research, 81(11), 3022-3035.

Klampatsa, A., Dimou, V., & Albelda, S. M. (2021). Mesothelin-targeted CAR-T cell therapy for solid tumors. Expert opinion on biological therapy, 21(4), 473-486.

Weidemann, S., Gagelmann, P., Gorbokon, N., Lennartz, M., Menz, A., Luebke, A. M., ... & Simon, R. (2021). Mesothelin expression in human tumors: a tissue microarray study on 12,679 tumors. Biomedicines, 9(4), 397.

Kaeding, A. J., Barwe, S. P., Gopalakrishnapillai, A., Ries, R. E., Alonzo, T. A., Gerbing, R. B., ... & Meshinchi, S. (2021). Mesothelin is a novel cell surface disease marker and potential therapeutic target in acute myeloid leukemia. Blood Advances, 5(9), 2350-2361.

Montemagno, C., Cassim, S., Pouyssegur, J., Broisat, A., & Pagès, G. (2020). From malignant progression to therapeutic targeting: current insights of mesothelin in pancreatic ductal adenocarcinoma. International Journal of Molecular Sciences, 21(11), 4067.

Shen, J., Sun, X., & Zhou, J. (2020). Insights into the role of mesothelin as a diagnostic and therapeutic target in ovarian carcinoma. Frontiers in Oncology, 10, 1263.

Hagemann, U. B., Ellingsen, C., Schuhmacher, J., Kristian, A., Mobergslien, A., Cruciani, V., ... & Cuthbertson, A. S. (2019). Mesothelin-targeted thorium-227 conjugate (MSLN-TTC): preclinical evaluation of a new targeted alpha therapy for mesothelin-positive cancers. Clinical Cancer Research, 25(15), 4723-4734.

Lv, J., & Li, P. (2019). Mesothelin as a biomarker for targeted therapy. Biomarker Research, 7(1), 1-18.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant MSLN protein, His Tag (CAT#: V2LY-0526-LY1603)

Human Recombinant MSLN protein, hFc Tag (CAT#: V2LY-0526-LY5544)

Human Recombinant MSLN protein, AF647 Conjugated, His Tag (CAT#: V2LY-0526-LY5545)

Human Recombinant MSLN protein, APC Conjugated, His Tag (CAT#: V2LY-0526-LY5546)

Human Recombinant MSLN protein, Biotin Conjugated, hFc & AVI Tag (CAT#: V2LY-0526-LY5547)

Human Recombinant MSLN protein, hFc Tag-1 (CAT#: V2LY-0526-LY5548)

Human Recombinant MSLN protein, Biotin Conjugated, hFc Tag (CAT#: V2LY-0526-LY5549)

Human Recombinant MSLN protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY5550)

Human Recombinant MSLN protein, His Tag (CAT#: V2LY-0526-LY5552)

Human Recombinant MSLN protein, His Tag-1 (CAT#: V2LY-0526-LY5553)

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Human Recombinant MSLN protein, hFc Tag-1 (CAT#: V2LY-0526-LY5548)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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