Human Recombinant MTSS1 protein, His & MBP Tag (V2LY-0526-LY5652)

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Basic Information

Expressed Host
E. coli
Protein Species
Human
Tag
His & MBP Tag
Protein Construction
This product is Human Recombinant MTSS1 protein, His & MBP Tag consist of Amino Acid: 1-250 and predicts a molecular mass of 71.8 kDa.
Molecule Mass
71.8 kDa
Sequence
Amino Acid: 1-250
Species
Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>80% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
MTSS1, I-BAR Domain Containing
Function
May be related to cancer progression or tumor metastasis in a variety of organ sites, most likely through an interaction with the actin cytoskeleton.
Biological Process
Actin cytoskeleton organization Source: UniProtKB
Adherens junction maintenance Source: GO_Central
Cell adhesion Source: UniProtKB
Cellular response to fluid shear stress Source: UniProtKB
Epithelial cell proliferation involved in renal tubule morphogenesis Source: UniProtKB
Glomerulus morphogenesis Source: UniProtKB
Microspike assembly Source: UniProtKB
Negative regulation of epithelial cell proliferation Source: UniProtKB
Nephron tubule epithelial cell differentiation Source: UniProtKB
Plasma membrane organization Source: InterPro
Positive regulation of actin filament bundle assembly Source: GO_Central
Renal tubule morphogenesis Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: UniProtKB
Cellular Location
Cytoskeleton

Wang, Y., Jia, Z., Liang, C., He, Y., Cong, M., Wu, Q., ... & Hu, G. (2023). MTSS1 curtails lung adenocarcinoma immune evasion by promoting AIP4-mediated PD-L1 monoubiquitination and lysosomal degradation. Cell Discovery, 9(1), 20.

Wu, M., Qiu, Q., Zhou, Q., Li, J., Yang, J., Zheng, C., ... & Lu, Y. (2022). circFBXO7/miR-96-5p/MTSS1 axis is an important regulator in the Wnt signaling pathway in ovarian cancer. Molecular Cancer, 21(1), 1-19.

Grandits, A. M., Nguyen, C. H., Schlerka, A., Hackl, H., Sill, H., Etzler, J., ... & Wieser, R. (2021). Downregulation of MTSS1 in acute myeloid leukemia is associated with a poor prognosis, chemotherapy resistance, and disease aggressiveness. Leukemia, 35(10), 2827-2839.

Grandits, A. M., & Wieser, R. (2021). Gene expression changes contribute to stemness and therapy resistance of relapsed acute myeloid leukemia: roles of SOCS2, CALCRL, MTSS1, and KDM6A. Experimental hematology, 99, 1-11.

Chen, J. X., Wang, Y. P., Zhang, X., Li, G. X., Zheng, K., & Duan, C. Z. (2020). lncRNA Mtss1 promotes inflammatory responses and secondary brain injury after intracerebral hemorrhage by targeting miR-709 in mice. Brain research bulletin, 162, 20-29.

Cong, M., Wang, Y., Yang, Y., Lian, C., Zhuang, X., Li, X., ... & Hu, G. (2020). MTSS1 suppresses mammary tumor-initiating cells by enhancing RBCK1-mediated p65 ubiquitination. Nature Cancer, 1(2), 222-234.

Chen, J., Huang, L., Zhu, Q., Wang, Z., & Tang, Z. (2020). MTSS1 hypermethylation is associated with prostate cancer progression. Journal of cellular physiology, 235(3), 2687-2697.

Hu, Y., Wang, F., Xu, F., Fang, K., Fang, Z., Shuai, X., ... & Zhao, G. (2020). A reciprocal feedback of Myc and lncRNA MTSS1-AS contributes to extracellular acidity-promoted metastasis of pancreatic cancer. Theranostics, 10(22), 10120.

Liu, K., Jiao, X. D., Hao, J. L., Qin, B. D., Wu, Y., Chen, W., ... & Zang, Y. S. (2019). MTSS1 inhibits metastatic potential and induces G2/M phase cell cycle arrest in gastric cancer. OncoTargets and therapy, 12, 5143.

Morley, M. P., Wang, X., Hu, R., Brandimarto, J., Tucker, N. R., Felix, J. F., ... & Cappola, T. P. (2019). Cardioprotective effects of MTSS1 enhancer variants. Circulation, 139(17), 2073-2076.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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