Human Recombinant MUC16 protein, ECD, His Tag-1 (V2LY-0526-LY2462)

Name: Human Recombinant MUC16 protein, ECD, His Tag-1
SKU: V2LY-0526-LY2462
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MUC16 protein, ECD, His Tag consist of Amino Acid: 13810-14451 and predicts a molecular mass of 73.27 kDa.

Molecule Mass

73.27 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 13810-14451

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Mucin 16, Cell Surface Associated

Function

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.

Biological Process

Cell adhesion Source: UniProtKB

Cellular Location

Extracellular space
Plasma membrane
Cell membrane
Note: May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

Topology

Extracellular: 1-14451
Helical: 14452-14472
Cytoplasmic: 14473-14507

PTM

Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.

Giamougiannis, P., Martin-Hirsch, P. L., & Martin, F. L. (2021). The evolving role of MUC16 (CA125) in the transformation of ovarian cells and the progression of neoplasia. Carcinogenesis, 42(3), 327-343.

Liu, J., Li, L., Luo, N., Liu, Q., Liu, L., Chen, D., ... & Xi, X. (2021). Inflammatory signals induce MUC16 expression in ovarian cancer cells via NF‑κB activation. Experimental and Therapeutic Medicine, 21(2), 1-1.

Crawford, A., Haber, L., Kelly, M. P., Vazzana, K., Canova, L., Ram, P., ... & Kirshner, J. R. (2019). A Mucin 16 bispecific T cell–engaging antibody for the treatment of ovarian cancer. Science translational medicine, 11(497), eaau7534.

Chen, X., Li, X., Wang, X., Zhu, Q., Wu, X., & Wang, X. (2019). MUC16 impacts tumor proliferation and migration through cytoplasmic translocation of P120-catenin in epithelial ovarian cancer cells: an original research. BMC cancer, 19(1), 1-12.

Felder, M., Kapur, A., Rakhmilevich, A. L., Qu, X., Sondel, P. M., Gillies, S. D., ... & Patankar, M. S. (2019). MUC16 suppresses human and murine innate immune responses. Gynecologic oncology, 152(3), 618-628.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant MUC16 protein, ECD, His Tag (CAT#: V2LY-0526-LY2461)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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