Human Recombinant MXRA8 protein, ECD, His Tag (V2LY-0526-LY5669)

Name: Human Recombinant MXRA8 protein, ECD, His Tag
SKU: V2LY-0526-LY5669
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant MXRA8 protein, ECD, His Tag consist of Amino Acid: 1-341 and predicts a molecular mass of 36.98 kDa.

Molecule Mass

36.98 kDa

Protein Domain

ECD

Verified

HPLC

Sequence

Amino Acid: 1-341

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥90% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

MATRIX REMODELING ASSOCIATED 8

Function

Transmembrane protein which can modulate activity of various signaling pathways, probably via binding to integrin ITGAV:ITGB3 (PubMed:22492581, PubMed:23386276).

Mediates heterophilic cell-cell interactions in vitro (By similarity).

Inhibits osteoclastogenesis downstream of TNFSF11/RANKL and CSF1, where it may function by attenuating signaling via integrin ITGB3 and MAP kinase p38 (By similarity).

Plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes (By similarity).

Stimulates formation of primary cilia in chondrocytes (By similarity).

Enhances expression of genes involved in the hedgehog signaling pathway in chondrocytes, including the hedgehog signaling molecule IHH; may also promote signaling via the PTHLH/PTHrP pathway (By similarity).

Plays a role in angiogenesis where it suppresses migration of endothelial cells and also promotes their apoptosis (PubMed:23386276).

Inhibits VEGF-induced activation of AKT and p38 MAP kinase in endothelial cells (PubMed:23386276).

Also inhibits VTN (vitronectin)-mediated integrin ITGAV:ITGB3 signaling and activation of PTK2/FAK (PubMed:23386276).

May play a role in the maturation and maintenance of the blood-brain barrier (By similarity).

(Microbial infection) Contributes to arthritogenic alphavirus pathogenesis and acts as a receptor for these viruses.

Biological Process

Cell adhesion Source: UniProtKB-KW
Establishment of glial blood-brain barrier Source: CAFA

Cellular Location

Cytoplasm
Nucleus
Plasma membrane
Cell membrane
cilium membrane
Other locations
tight junction
Note: Primarily localizes to the cell membrane (PubMed:23386276). Detected in the cilium of primary chondrocytes (By similarity). Highly expressed at areas of cell-cell contact and may localize to tight junctions (By similarity). Also found in the nucleus where it is detected in the soluble (as opposed to chromatin-bound) fraction (PubMed:23386276).

Topology

Extracellular: 20-341
Helical: 342-362
Cytoplasmic: 363-442

Zimmerman, O., Zimmerman, M. I., Raju, S., Nelson, C. A., Errico, J. M., Madden, E. A., ... & Diamond, M. S. (2023). Vertebrate-class-specific binding modes of the alphavirus receptor MXRA8. Cell, 186(22), 4818-4833.

Simpson, K. E., Staikos, C. A., Watson, K. L., & Moorehead, R. A. (2023). Loss of MXRA8 Delays Mammary Tumor Development and Impairs Metastasis. International Journal of Molecular Sciences, 24(18), 13730.

Ng, W. H., Ling, Z. L., Kueh, A. J., Herold, M. J., West, N. P., King, N. J., ... & Liu, X. (2023). Role of MXRA8 in Ross River Virus Disease Pathogenesis. Mbio, 14(2), e00588-23.

Feng, F., Bouma, E. M., Hu, G., Zhu, Y., Yu, Y., Smit, J. M., ... & Zhang, R. (2023). Colocalization of Chikungunya Virus with Its Receptor MXRA8 during Cell Attachment, Internalization, and Membrane Fusion. Journal of Virology, 97(5), e01557-22.

Yin, P., & Kielian, M. (2021). BHK-21 cell clones differ in chikungunya virus infection and MXRA8 receptor expression. Viruses, 13(6), 949.

Powell, L. A., Miller, A., Fox, J. M., Kose, N., Klose, T., Kim, A. S., ... & Crowe, J. E. (2020). Human mAbs broadly protect against arthritogenic alphaviruses by recognizing conserved elements of the Mxra8 receptor-binding site. Cell host & microbe, 28(5), 699-711.

Kim, A. S., Zimmerman, O., Fox, J. M., Nelson, C. A., Basore, K., Zhang, R., ... & Diamond, M. S. (2020). An evolutionary insertion in the Mxra8 receptor-binding site confers resistance to alphavirus infection and pathogenesis. Cell host & microbe, 27(3), 428-440.

Basore, K., Kim, A. S., Nelson, C. A., Zhang, R., Smith, B. K., Uranga, C., ... & Fremont, D. H. (2019). Cryo-EM structure of chikungunya virus in complex with the Mxra8 receptor. Cell, 177(7), 1725-1737.

Zhang, R., Earnest, J. T., Kim, A. S., Winkler, E. S., Desai, P., Adams, L. J., ... & Diamond, M. S. (2019). Expression of the Mxra8 receptor promotes alphavirus infection and pathogenesis in mice and Drosophila. Cell reports, 28(10), 2647-2658.

Song, H., Zhao, Z., Chai, Y., Jin, X., Li, C., Yuan, F., ... & Gao, G. F. (2019). Molecular basis of arthritogenic alphavirus receptor MXRA8 binding to chikungunya virus envelope protein. Cell, 177(7), 1714-1724.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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