Human Recombinant NECTIN2 protein, ECD, Biotin Conjugated, hFc & AVI Tag (V2LY-0526-LY5716)

Name: Human Recombinant NECTIN2 protein, ECD, Biotin Conjugated, hFc & AVI Tag
SKU: V2LY-0526-LY5716
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc & AVI Tag

Protein Construction

This product is Human Recombinant NECTIN2 protein, ECD, Biotin Conjugated, hFc & AVI Tag consist of Amino Acid: 1-360 and predicts a molecular mass of 64.08 kDa.

Molecule Mass

64.08 kDa

Protein Domain

ECD

Verified

HPLC

Conjugates

Biotin

Sequence

Amino Acid: 1-360

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE. ≥95% as determined by SEC-HPLC.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Glutathione, EDTA, DTT, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

nectin cell adhesion molecule 2

Function

Modulator of T-cell signaling. Can be either a costimulator of T-cell function, or a coinhibitor, depending on the receptor it binds to. Upon binding to CD226, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Upon interaction with PVRIG, inhibits T-cell proliferation. These interactions are competitive (PubMed:26755705).

Probable cell adhesion protein (PubMed:9657005).

(Microbial infection) Acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).

Biological Process

Acrosome assembly Source: GO_Central
Adhesion of symbiont to host Source: BHF-UCL
Cell part morphogenesis Source: Ensembl
Cilium organization Source: Ensembl
Coreceptor-mediated virion attachment to host cell Source: BHF-UCL
Cytoskeleton organization Source: GO_Central
Establishment of mitochondrion localization Source: GO_Central
Fertilization Source: Ensembl
Fusion of virus membrane with host plasma membrane Source: BHF-UCL
Homophilic cell adhesion via plasma membrane adhesion molecules Source: BHF-UCL
Positive regulation of immunoglobulin mediated immune response Source: BHF-UCL
Positive regulation of mast cell activation Source: BHF-UCL
Positive regulation of natural killer cell mediated cytotoxicity Source: BHF-UCL
Positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: BHF-UCL
Positive regulation of T cell receptor signaling pathway Source: UniProtKB
Regulation of viral entry into host cell Source: CACAO
Spermatid development Source: BHF-UCL
Spermatid nucleus differentiation Source: Ensembl
Sperm mitochondrion organization Source: Ensembl
Susceptibility to natural killer cell mediated cytotoxicity Source: BHF-UCL
Susceptibility to T cell mediated cytotoxicity Source: BHF-UCL

Cellular Location

Cell membrane

Topology

Extracellular: 32-360
Helical: 361-381
Cytoplasmic: 382-538

Thongsom, S., Aksorn, N., Petsri, K., Roytrakul, S., Sriratanasak, N., Wattanathana, W., & Chanvorachote, P. (2022). Analysis of Protein–Protein Interactions Identifies NECTIN2 as a Target of N, N-Bis (5-Ethyl-2-hydroxybenzyl) Methylamine for Inhibition of Lung Cancer Metastasis. Cancer Genomics & Proteomics, 19(5), 624-635.

Mizutani, K., Miyata, M., Shiotani, H., Kameyama, T., & Takai, Y. (2022). Nectin-2 in general and in the brain. Molecular and Cellular Biochemistry, 1-14.

Ogawa, H., Fujikura, D., Namba, H., Yamashita, N., Honda, T., & Yamada, M. (2022). Nectin-2 acts as a viral entry mediated molecule that binds to human herpesvirus 6B glycoprotein B. Viruses, 14(1), 160.

Ak, N., Serilmez, M., Üçüncü, M. Z., Bademler, S., & Vatansever, S. (2021). Nectin-2 and nectin-4 adhesion molecules in patients with breast cancer. Türk Onkoloji Dergisi-Turkish Journal of Oncology.

Erturk, K., Karaman, S., Dagoglu, N., Serilmez, M., Duranyildiz, D., & Tas, F. (2019). Serum nectin-2 and nectin-4 are diagnostic in lung cancer: which is superior?. Wiener klinische Wochenschrift, 131, 419-426.

Bekes, I., Löb, S., Holzheu, I., Janni, W., Baumann, L., Wöckel, A., & Wulff, C. (2019). Nectin‐2 in ovarian cancer: How is it expressed and what might be its functional role?. Cancer Science, 110(6), 1872-1882.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Cynomolgus Recombinant NECTIN2 protein, ECD, His Tag (CAT#: V2LY-0526-LY1606)

Human Recombinant NECTIN2 protein, ECD, His Tag (CAT#: V2LY-0526-LY5717)

Human Recombinant NECTIN2 protein, hFc Tag (CAT#: V2LY-0526-LY5718)

Human Recombinant NECTIN2 protein, hFc Tag-1 (CAT#: V2LY-0526-LY5719)

Human Recombinant NECTIN2 protein, Biotin Conjugated, His & AVI Tag (CAT#: V2LY-0526-LY5720)

Human Recombinant NECTIN2 protein (CAT#: V2LY-0526-LY5721)

Mouse Recombinant NECTIN2 protein, His Tag (CAT#: V2LY-0526-LY8691)

Rat Recombinant NECTIN2 protein, His Tag (CAT#: V2LY-0526-LY9528)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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