Human Recombinant NLN protein, His Tag (V2LY-0526-LY5767)

Name: Human Recombinant NLN protein, His Tag
SKU: V2LY-0526-LY5767
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant NLN protein, His Tag consist of Amino Acid: 38-704 and predicts a molecular mass of 78.7 kDa.

Molecule Mass

78.7 kDa

Sequence

Amino Acid: 38-704

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>90% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Neurolysin

Function

Hydrolyzes oligopeptides such as neurotensin, bradykinin and dynorphin A.

Biological Process

G protein-coupled receptor signaling pathwayTAS:Reactome
Peptide metabolic processManual Assertion Based On ExperimentIBA:GO_Central
ProteolysisManual Assertion Based On ExperimentIBA:GO_Central
Regulation of gluconeogenesisIEA:Ensembl
Regulation of skeletal muscle fiber differentiationIEA:Ensembl

Cellular Location

Mitochondrion intermembrane space
Cytoplasm

Caprioli, B., Eichler, R. A., Silva, R. N., Martucci, L. F., Reckziegel, P., & Ferro, E. S. (2023). Neurolysin Knockout Mice in a Diet-Induced Obesity Model. International Journal of Molecular Sciences, 24(20), 15190.

Esfahani, S. H., Abbruscato, T. J., Trippier, P. C., & Karamyan, V. T. (2022). Small molecule neurolysin activators, potential multi-mechanism agents for ischemic stroke therapy. Expert Opinion on Therapeutic Targets, 26(5), 401-404.

Karamyan, V. T. (2021). The role of peptidase neurolysin in neuroprotection and neural repair after stroke. Neural Regeneration Research, 16(1), 21.

Jayaraman, S., Kocot, J., Esfahani, S. H., Wangler, N. J., Uyar, A., Mechref, Y., ... & Karamyan, V. T. (2021). Identification and characterization of two structurally related dipeptides that enhance catalytic efficiency of neurolysin. Journal of Pharmacology and Experimental Therapeutics, 379(2), 191-202.

Jayaraman, S., Al Shoyaib, A., Kocot, J., Villalba, H., Alamri, F. F., Rashid, M., ... & Karamyan, V. T. (2020). Peptidase neurolysin functions to preserve the brain after ischemic stroke in male mice. Journal of neurochemistry, 153(1), 120-137.

Mirali, S., Botham, A., Voisin, V., Xu, C., St-Germain, J., Sharon, D., ... & Schimmer, A. D. (2020). The mitochondrial peptidase, neurolysin, regulates respiratory chain supercomplex formation and is necessary for AML viability. Science Translational Medicine, 12(538), eaaz8264.

Karamyan, V. T. (2019). Peptidase neurolysin is an endogenous cerebroprotective mechanism in acute neurodegenerative disorders. Medical hypotheses, 131, 109309.

Mirali, S., Aaron, B., Voisin, V., Xu, C., St-Germain, J., Sharon, D., ... & Schimmer, A. D. (2019). The Mitochondrial Protease, Neurolysin (NLN), Regulates Respiratory Chain Complex and Supercomplex Formation and Is Necessary for AML Viability. Blood, 134, 729.

Jayaraman, S., Rashid, M., Wangler, N. J., Villalba, H., German, N., Arumugam, T. V., ... & Karamyan, V. T. (2019). Peptidase Neurolysin Functions to Preserve the Brain After Ischemic Stroke in Mice. Stroke, 50(Suppl_1), A157-A157.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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