Human Recombinant PI4KB, Active protein, GST Tag (V2LY-0526-LY6168)

Name: Human Recombinant PI4KB, Active protein, GST Tag
SKU: V2LY-0526-LY6168
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant PI4KB, Active protein, GST Tag consist of Amino Acid: Full Length and predicts a molecular mass of 120 kDa.

Molecule Mass

120 kDa

Sequence

Amino Acid: Full Length

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Phosphatidylinositol 4-Kinase Beta

Function

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).
(Microbial infection) Plays an essential role in Aichi virus RNA replication (PubMed:22124328, PubMed:27989622, PubMed:22258260).
Recruited by ACBD3 at the viral replication sites (PubMed:22124328, PubMed:27989622).
(Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV.

Biological Process

Lysosome organizationManual Assertion Based On ExperimentIDA:MGI
Phosphatidylinositol biosynthetic processTAS:Reactome
Phosphatidylinositol phosphate biosynthetic processManual Assertion Based On ExperimentIBA:GO_Central
Phosphatidylinositol-mediated signalingManual Assertion Based On ExperimentIBA:GO_Central
Receptor-mediated endocytosisManual Assertion Based On ExperimentTAS:ProtInc
Signal transductionManual Assertion Based On ExperimentTAS:ProtInc

Cellular Location

Endomembrane system
Mitochondrion outer membrane
Rough endoplasmic reticulum membrane
Golgi apparatus
Golgi apparatus membrane
Cytoplasm, perinuclear region
Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex. Recruited to the Golgi apparatus membrane by ACBD3 (PubMed:24672044, PubMed:27009356, PubMed:28289207).
GGA2 is also involved in the recruitment (PubMed:28289207).

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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