Human Recombinant RBBP4 protein, His Tag (V2LY-0526-LY6469)

Name: Human Recombinant RBBP4 protein, His Tag
SKU: V2LY-0526-LY6469
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant RBBP4 protein, His Tag consist of Amino Acid: 1-425 and predicts a molecular mass of 50 kDa.

Molecule Mass

50 kDa

Sequence

Amino Acid: 1-425

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

RB Binding Protein 4, Chromatin Remodeling Factor

Function

Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Biological Process

Biological Process brain development1 PublicationIC:ComplexPortal
Biological Process cell cycleIEA:UniProtKB-KW
Biological Process chromatin assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process chromatin remodelingManual Assertion Based On ExperimentIDA:HGNC-UCL
Biological Process DNA replicationIEA:UniProtKB-KW
Biological Process DNA replication-dependent chromatin assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process DNA replication-independent chromatin assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process histone deacetylationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process negative regulation of cell migration1 PublicationIC:ComplexPortal
Biological Process negative regulation of cell population proliferationManual Assertion Based On ExperimentTAS:ProtInc
Biological Process negative regulation of stem cell population maintenance1 PublicationIC:ComplexPortal
Biological Process negative regulation of transcription by RNA polymerase II2 PublicationsIC:ComplexPortal
Biological Process negative regulation of transcription, DNA-templated1 PublicationIC:ComplexPortal
Biological Process negative regulation of transforming growth factor beta receptor signaling pathway1 PublicationIC:ComplexPortal
Biological Process positive regulation of stem cell population maintenance1 PublicationIC:ComplexPortal
Biological Process positive regulation of transcription, DNA-templated1 PublicationIC:ComplexPortal
Biological Process regulation of cell fate specification1 PublicationIC:ComplexPortal
Biological Process regulation of stem cell differentiation1 PublicationIC:ComplexPortal
Biological Process regulation of transcription, DNA-templatedManual Assertion Based On ExperimentIDA:ComplexPortal

Cellular Location

Nucleus
Chromosome, telomere
Localizes to chromatin as part of the PRC2 complex.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant RBBP4 protein, GST Tag (CAT#: V2LY-0526-LY6468)

Mouse Recombinant RBBP4 protein, His Tag (CAT#: V2LY-0526-LY8824)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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