Human Recombinant RNF4 protein, GST Tag (V2LY-0526-LY6565)

Name: Human Recombinant RNF4 protein, GST Tag
SKU: V2LY-0526-LY6565
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

GST Tag

Protein Construction

This product is Human Recombinant RNF4 protein, GST Tag consist of Amino Acid: 1-190 and predicts a molecular mass of 52 kDa.

Molecule Mass

52 kDa

Sequence

Amino Acid: 1-190

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

PBS

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Ring Finger Protein 4

Function

E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.

Biological Process

Biological Process positive regulation of transcription by RNA polymerase IIManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of transcription, DNA-templatedISS:UniProtKB
Biological Process proteasome-mediated ubiquitin-dependent protein catabolic processManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein autoubiquitinationISS:UniProtKB
Biological Process protein K11-linked ubiquitinationISS:UniProtKB
Biological Process protein K48-linked ubiquitinationISS:UniProtKB
Biological Process protein K6-linked ubiquitinationISS:UniProtKB
Biological Process protein K63-linked ubiquitinationISS:UniProtKB
Biological Process regulation of kinetochore assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process regulation of spindle assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process response to arsenic-containing substanceManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

Cytoplasm
Nucleus
Nucleus, PML body
Nucleus, nucleoplasm

PTM

Sumoylated; conjugated by one or two SUMO1 moieties.
Autoubiquitinated.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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