Human Recombinant S100A10 protein, His Tag (V2LY-0526-LY6639)

Name: Human Recombinant S100A10 protein, His Tag
SKU: V2LY-0526-LY6639
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

E. coli

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant S100A10 protein, His Tag consist of Amino Acid: 2-97 and predicts a molecular mass of 12.6 kDa.

Molecule Mass

12.6 kDa

Sequence

Amino Acid: 2-97

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, Tween80, Trehalose

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

S100 Calcium Binding Protein A10

Function

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

Biological Process

Biological Process membrane raft assemblyManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process mRNA transcription by RNA polymerase IIIEA:Ensembl
Biological Process positive regulation of bindingIEA:Ensembl
Biological Process positive regulation of exocytosis1 PublicationIC:ComplexPortal
Biological Process positive regulation of focal adhesion assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of GTPase activityManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of plasma membrane repair1 PublicationIC:ComplexPortal
Biological Process positive regulation of plasminogen activationManual Assertion Based On ExperimentIDA:ComplexPortal
Biological Process positive regulation of stress fiber assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of substrate adhesion-dependent cell spreadingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of transcription by RNA polymerase IIIEA:Ensembl
Biological Process protein localization to plasma membraneManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process regulation of neurogenesisIEA:Ensembl
Biological Process vesicle budding from membraneManual Assertion Based On ExperimentIDA:UniProtKB

Cellular Location

AnxA2-p11 complex
collagen-containing extracellular matrix
cytoplasm
extracellular exosome
extracellular region
extracellular space
extrinsic component of plasma membrane
membrane
nuclear matrix
plasma membrane
plasma membrane protein complex
RNA polymerase II transcription regulator complex

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human S100A10 ELISA Kit (CAT#: V2LY-0626-LY3952)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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