Human Recombinant SELP protein, hFc Tag (V2LY-0526-LY6363)

Name: Human Recombinant SELP protein, hFc Tag
SKU: V2LY-0526-LY6363
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

hFc Tag

Protein Construction

This product is Human Recombinant SELP protein, hFc Tag consist of Amino Acid: 1-771 and predicts a molecular mass of 107 kDa.

Molecule Mass

107 kDa

Sequence

Amino Acid: 1-771

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>85% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Selectin P

Function

Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.

Biological Process

Biological Process calcium-dependent cell-cell adhesion via plasma membrane cell adhesion moleculesManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process calcium-mediated signaling using intracellular calcium sourceManual Assertion Based On ExperimentIGI:UniProtKB
Biological Process cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process defense response to Gram-negative bacterium1 PublicationIC:BHF-UCL
Biological Process heterophilic cell-cell adhesion via plasma membrane cell adhesion moleculesManual Assertion Based On ExperimentIBA:GO_Central
Biological Process inflammatory responseIEA:Ensembl
Biological Process leukocyte cell-cell adhesionManual Assertion Based On ExperimentIDA:BHF-UCL
Biological Process leukocyte tethering or rollingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process positive regulation of leukocyte migrationIEA:Ensembl
Biological Process positive regulation of leukocyte tethering or rollingISS:UniProtKB
Biological Process positive regulation of phosphatidylinositol 3-kinase signalingManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process positive regulation of platelet activationBy SimilarityISS:BHF-UCL
Biological Process regulation of integrin activationManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process response to cytokineManual Assertion Based On ExperimentIBA:GO_Central
Biological Process response to lipopolysaccharide1 PublicationIC:BHF-UCL

Cellular Location

Cell membrane

Involvement in disease

Ischemic stroke (ISCHSTR):
A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

Topology

Extracellular: 42-771
Helical: 772-795
Cytoplasmic: 796-830

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant SELP protein, ECD, His Tag (CAT#: V2LY-0526-LY6362)

Mouse Recombinant SELP protein, hFc Tag (CAT#: V2LY-0526-LY8812)

Mouse Recombinant SELP protein, His Tag (CAT#: V2LY-0526-LY8813)

Rat Recombinant SELP protein, hFc Tag (CAT#: V2LY-0526-LY9564)

Rat Recombinant SELP protein, His Tag (CAT#: V2LY-0526-LY9565)

Rhesus Recombinant SELP protein, hFc Tag (CAT#: V2LY-0526-LY9783)

Rhesus Recombinant SELP protein, His Tag (CAT#: V2LY-0526-LY9784)

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Rat Recombinant SELP protein, hFc Tag (CAT#: V2LY-0526-LY9564)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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