Human Recombinant SELPLG protein, His Tag (V2LY-0526-LY6368)

Name: Human Recombinant SELPLG protein, His Tag
SKU: V2LY-0526-LY6368
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant SELPLG protein, His Tag consist of Amino Acid: 1-295 and predicts a molecular mass of 30.5 kDa.

Molecule Mass

30.5 kDa

Sequence

Amino Acid: 1-295

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tirs, NaCl, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Selectin P Ligand

Function

A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
(Microbial infection) Acts as a receptor for enterovirus 71.

Biological Process

Biological Process cell adhesionManual Assertion Based On ExperimentTAS:ProtInc
Biological Process cellular response to interleukin-6Manual Assertion Based On ExperimentIDA:MGI
Biological Process hemopoiesisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process leukocyte adhesive activationISS:UniProtKB
Biological Process leukocyte migrationManual Assertion Based On ExperimentIDA:CAFA
Biological Process leukocyte tethering or rollingManual Assertion Based On ExperimentIDA:CAFA

Cellular Location

Membrane

Topology

Extracellular: 18-320
Helical: 321-341
Cytoplasmic: 342-412

PTM

Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant SELPLG protein, His & hFc Tag (CAT#: V2LY-0526-LY6367)

Human Recombinant SELPLG protein, Fc Tag (CAT#: V2LY-0526-LY6369)

Mouse Recombinant SELPLG protein (CAT#: V2LY-0526-LY8814)

Mouse Recombinant SELPLG protein, hFc Tag (CAT#: V2LY-0526-LY8815)

Human SELPLG ELISA Kit (CAT#: V2LY-0626-LY5143)

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Rat Anti-SELPLG Recombinant Antibody (A1424) (CAT#: CBMAB-AP11982LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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