Human Recombinant SYAP1 protein, His Tag (V2LY-0526-LY7023)

Name: Human Recombinant SYAP1 protein, His Tag
SKU: V2LY-0526-LY7023
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant SYAP1 protein, His Tag consist of Amino Acid: 1-352 and predicts a molecular mass of 41.39 kDa.

Molecule Mass

41.39 kDa

Sequence

Amino Acid: 1-352

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

SYAP1

Function

Plays a role in adipocyte differentiation by promoting mTORC2-mediated phosphorylation of AKT1 at 'Ser-473' after growth factor stimulation (PubMed:23300339).

Biological Process

Biological Process cell differentiationIEA:UniProtKB-KW
Biological Process cellular response to epidermal growth factor stimulusIMP:UniProtKB1 Publication
Biological Process cellular response to insulin stimulusIMP:UniProtKB1 Publication
Biological Process cellular response to insulin-like growth factor stimulusIMP:UniProtKB1 Publication
Biological Process cellular response to platelet-derived growth factor stimulusIMP:UniProtKB1 Publication
Biological Process positive regulation of fat cell differentiationIMP:UniProtKB1 Publication
Biological Process positive regulation of protein serine/threonine kinase activityIMP:UniProtKB1 Publication
Biological Process regulation of short-term neuronal synaptic plasticityIBA:GO_Central1 Publication
Biological Process TORC2 signalingIMP:UniProtKB1 Publication

Cellular Location

Cytoplasm, perinuclear region
Golgi apparatus
Perikaryon
Cell projection, axon
Cell projection, dendrite
Cell projection, growth cone
Presynaptic cell membrane
Postsynaptic cell membrane
Membrane
Localizes to cholinergic neuromuscular junctions and in actin-rich growth cone regions (By similarity).
Membrane-associated in a epidermal growth factor (EGF)-dependent manner (PubMed:23300339).

PTM

Phosphorylated. Phosphorylation increases in a mTORC2-mediated manner in response to epidermal growth factor (EGF) stimulation.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-SYAP1 Recombinant Antibody (CBXS-5536) (CAT#: CBMAB-S2743-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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