Human Recombinant TOP1 protein, Biotin Conjugated, His Tag (V2LY-0526-LY7318)

Name: Human Recombinant TOP1 protein, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY7318
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant TOP1 protein, Biotin Conjugated, His Tag consist of Amino Acid: 1-765 and predicts a molecular mass of 93.06 kDa.

Molecule Mass

93.06 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-765

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

≥90% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

TOP1

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Biological Process

Biological Process chromatin remodeling Source:UniProtKB1 Publication
Biological Process chromosome segregation Source:GO_Central1 Publication
Biological Process circadian regulation of gene expression Source:UniProtKB1 Publication
Biological Process circadian rhythm Source:UniProtKB1 Publication
Biological Process DNA replication Source:GO_Central1 Publication
Biological Process DNA topological change Source:UniProtKB1 Publication
Biological Process embryonic cleavage Source:Ensembl
Biological Process peptidyl-serine phosphorylation Source:CAFA1 Publication
Biological Process phosphorylation Source:UniProtKB1 Publication
Biological Process programmed cell death Source:UniProtKB1 Publication
Biological Process response to xenobiotic stimulus Source:UniProtKB1 Publication

Cellular Location

Nucleus, nucleolus
Nucleus, nucleoplasm
Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli.

Involvement in disease

A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.

PTM

Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant TOP1 protein, His Tag (CAT#: V2LY-0526-LY7316)

Human Recombinant TOP1 protein, His Tag-1 (CAT#: V2LY-0526-LY7317)

Mouse Recombinant TOP1 protein, His Tag (CAT#: V2LY-0526-LY9002)

Mouse Recombinant TOP1 protein, His Tag-1 (CAT#: V2LY-0526-LY9003)

Mouse Top1 ELISA Kit (CAT#: V2LY-0626-LY2320)

Human TOP1 ELISA Kit (CAT#: V2LY-0626-LY3882)

Related Products

Mouse Anti-TOP1 Recombinant Antibody (CBYJT-4192) (CAT#: CBMAB-T3646-YJ)

Mouse Anti-TOP1 Recombinant Antibody (CBYJT-4196) (CAT#: CBMAB-T3651-YJ)

Mouse Anti-TOP1 Recombinant Antibody (AK5) (CAT#: CBMAB-D2343-YC)

Human Recombinant TOP1 protein, His Tag-1 (CAT#: V2LY-0526-LY7317)

Mouse Anti-TOP1 Recombinant Antibody (CBYJT-4197) (CAT#: CBMAB-T3652-YJ)

Mouse Anti-TOP1 Recombinant Antibody (CBT4743) (CAT#: V2LY-0625-LY1932)

Rabbit Anti-TOP1 Recombinant Antibody (CBYJT-4198) (CAT#: CBMAB-T3653-YJ)

Mouse Anti-TOP1 Recombinant Antibody (CBYJT-4194) (CAT#: CBMAB-T3649-YJ)

Mouse Top1 ELISA Kit (CAT#: V2LY-0626-LY2320)

Mouse Anti-TOP1 Recombinant Antibody (1291CT875.142.166) (CAT#: CBMAB-T3645-YJ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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