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TOP1

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq]
Full Name
TOP1
Function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
Biological Process
Biological Process chromatin remodeling Source:UniProtKB1 Publication
Biological Process chromosome segregation Source:GO_Central1 Publication
Biological Process circadian regulation of gene expression Source:UniProtKB1 Publication
Biological Process circadian rhythm Source:UniProtKB1 Publication
Biological Process DNA replication Source:GO_Central1 Publication
Biological Process DNA topological change Source:UniProtKB1 Publication
Biological Process embryonic cleavage Source:Ensembl
Biological Process peptidyl-serine phosphorylation Source:CAFA1 Publication
Biological Process phosphorylation Source:UniProtKB1 Publication
Biological Process programmed cell death Source:UniProtKB1 Publication
Biological Process response to xenobiotic stimulus Source:UniProtKB1 Publication
Cellular Location
Nucleus, nucleolus
Nucleus, nucleoplasm
Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli.
Involvement in disease
A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.
PTM
Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.

Anti-TOP1 antibodies

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Target: TOP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: AK5
Application*: WB
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 1A1
Application*: WB, E
Target: TOP1
Host: Mouse
Antibody Isotype: IgG2b
Specificity: Human
Clone: 11BA50
Application*: E, WB
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBYJT-4191
Application*: IH
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 1291CT875.142.166
Application*: E, WB, F
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBYJT-4192
Application*: E, WB
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYJT-4193
Application*: IF, IP, WB
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYJT-4194
Application*: WB
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYJT-4195
Application*: WB
Target: TOP1
Host: Mouse
Antibody Isotype: IgM
Specificity: Human
Clone: CBYJT-4196
Application*: WB
Target: TOP1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYJT-4197
Application*: WB
Target: TOP1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: CBYJT-4198
Application*: WB, P, F, IC, IF
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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