Mouse Anti-TOP1 Recombinant Antibody (CBT4743) (V2LY-0625-LY1932)

Name: Mouse Anti-TOP1 Recombinant Antibody (CBT4743)
SKU: V2LY-0625-LY1932
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-TOP1 Recombinant Antibody (CBT4743) (V2LY-0625-LY1932) in WB

Mouse Anti-TOP1 Recombinant Antibody (CBT4743) (V2LY-0625-LY1932) in IF

Mouse Anti-TOP1 Recombinant Antibody (CBT4743) (V2LY-0625-LY1932) in FC

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBT4743

Application

WB, ICC, FC

Immunogen

Purified recombinant fragment of human TOP1 expressed in E. Coli.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ICC	1:100-1:400
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

TOP1

Entrez Gene ID

7150

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).
Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Biological Process

Biological Process chromatin remodeling Source:UniProtKB1 Publication
Biological Process chromosome segregation Source:GO_Central1 Publication
Biological Process circadian regulation of gene expression Source:UniProtKB1 Publication
Biological Process circadian rhythm Source:UniProtKB1 Publication
Biological Process DNA replication Source:GO_Central1 Publication
Biological Process DNA topological change Source:UniProtKB1 Publication
Biological Process embryonic cleavage Source:Ensembl
Biological Process peptidyl-serine phosphorylation Source:CAFA1 Publication
Biological Process phosphorylation Source:UniProtKB1 Publication
Biological Process programmed cell death Source:UniProtKB1 Publication
Biological Process response to xenobiotic stimulus Source:UniProtKB1 Publication

Cellular Location

Nucleus, nucleolus
Nucleus, nucleoplasm
Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumoylated forms found in both nucleoplasm and nucleoli.

Involvement in disease

A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98.

PTM

Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment.
Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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